Linked Life Data

10623835

Source:http://linkedlifedata.com/resource/pubmed/id/10623835

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2000-2-10
pubmed:databankReference
pubmed:abstractText
The three CD3 components of the TCR complex are encoded as clustered genes in mammals. The evolution of such a multimeric complex is likely to occur stepwise. The chicken CD3 cluster was entirely sequenced, and, in contrast to mammals, only two chicken CD3 genes were found to be physically linked to the unrelated genes HZW10 and epithelial V-like Ag flanking both sides of the CD3 cluster. Biochemical analyses of CD3 immunoprecipitates confirmed the presence of only two CD3 proteins and revealed an essential role for CD3gammadelta glycosylation during assembly. Functional analyses indicated that the chicken TCR/CD3 complex was efficiently down-regulated by phorbol ester treatment, demonstrating the integrity of a CD3gamma-like cytoplasmic internalization motif. These data argue for a stepwise CD3 evolution, with major differences in the TCR/CD3 structure between mammalian and nonmammalian vertebrates setting a basis for the understanding of the CD3 phylogeny and proving the ancestral nature of the CD3gammadelta protein.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
164
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
879-83
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Evidence for a stepwise evolution of the CD3 family.
pubmed:affiliation
Institute for Animal Physiology, Munich, Germany. goebel@tiph.vetmed.uni-muenchen.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't