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rdf:type |
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lifeskim:mentions |
umls-concept:C0006685,
umls-concept:C0009015,
umls-concept:C0017262,
umls-concept:C0018787,
umls-concept:C0086418,
umls-concept:C0185117,
umls-concept:C0330390,
umls-concept:C0439097,
umls-concept:C0596901,
umls-concept:C0679729,
umls-concept:C1280500,
umls-concept:C1547348,
umls-concept:C1705241,
umls-concept:C1711351,
umls-concept:C2003941,
umls-concept:C2911684
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pubmed:issue |
1
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pubmed:dateCreated |
2000-2-8
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pubmed:databankReference |
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pubmed:abstractText |
Expression and membrane localization of an epitope-tagged human Ca(2+) channel alpha(1C) subunit were monitored in Xenopus oocytes by confocal microscopy and electrophysiological recording. When alpha(2)/delta and beta(2a) were separately coexpressed with the alpha(1C) subunit, assessment by confocal microscopy showed an 86 and 225% increase of the channel density, respectively. Simultaneous coexpression of alpha(2)/delta and beta(2a) subunits resulted in a cooperative (470%) increase. Electrophysiological measurements performed in parallel revealed that the current augmentation by the alpha(2)/delta subunit is totally attributable to an increase in channel density, whereas the beta(2a) subunit, in addition to increasing channel density, also facilitates channel opening.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2000 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
7
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pubmed:volume |
267
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
156-63
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10623591-Amino Acid Sequence,
pubmed-meshheading:10623591-Animals,
pubmed-meshheading:10623591-Barium,
pubmed-meshheading:10623591-Calcium Channels,
pubmed-meshheading:10623591-Cloning, Molecular,
pubmed-meshheading:10623591-Cloning, Organism,
pubmed-meshheading:10623591-Heart,
pubmed-meshheading:10623591-Humans,
pubmed-meshheading:10623591-Macromolecular Substances,
pubmed-meshheading:10623591-Membrane Potentials,
pubmed-meshheading:10623591-Mice,
pubmed-meshheading:10623591-Molecular Sequence Data,
pubmed-meshheading:10623591-Myocardium,
pubmed-meshheading:10623591-Oocytes,
pubmed-meshheading:10623591-Protein Structure, Quaternary,
pubmed-meshheading:10623591-Rabbits,
pubmed-meshheading:10623591-Rats,
pubmed-meshheading:10623591-Recombinant Proteins,
pubmed-meshheading:10623591-Sequence Alignment,
pubmed-meshheading:10623591-Sequence Homology, Amino Acid,
pubmed-meshheading:10623591-Xenopus,
pubmed-meshheading:10623591-Xenopus laevis
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pubmed:year |
2000
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pubmed:articleTitle |
Cloning of the beta(2a) subunit of the voltage-dependent calcium channel from human heart: cooperative effect of alpha(2)/delta and beta(2a) on the membrane expression of the alpha(1C) subunit.
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pubmed:affiliation |
Institute of Molecular Pharmacology, Department of Cell Biology, University of Cincinnati, College of Medicine, 231 Bethesda Avenue, Cincinnati, Ohio, 45267-0828, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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