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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2000-2-2
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pubmed:abstractText |
Hypericin (HY) is a powerful photo-inducer of apoptosis in Jurkat cells as measured by caspase-3 activation, cell shrinkage, phosphatidylserine (PS) exposure and the appearance of hypoploid DNA. These processes are preceded by rapid Bcl-2-independent mitochondrial transmembrane depolarization and a drop in cytoplasmic pH. Pre-incubation of cells with inhibitors of the mitochondrial permeability transition pore, such as cyclosporin A or bongkrekic acid, does not protect cells from mitochondrial membrane potential (deltapsim) decrease. However, monitoring of mitochondrial entrapped calcein by confocal fluorescence imaging gives clear evidence of HY photo-induced mitochondrial permeability. This should be considered as the result of a non-specific alteration of mitochondrial membrane integrity brought about by lipid peroxidation. Nevertheless, synthesis of the anti-apoptotic protein Bcl-2 appears to delay the subsequent time course of PS exposure and to reduce caspase-3 activation and the fraction of cells which become hypoploid. We interpret this partially protective effect as the consequence of a direct interaction of Bcl-2 with cytosolic cytochrome c previously released from mitochondria upon deltapsim decrease and/or of Bcl-2 inhibition of the deleterious retro-effect of caspase-3 on the mitochondrial permeability transition pore and/or the mitochondrial membrane components.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bongkrekic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/CASP3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Casp3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclosporine,
http://linkedlifedata.com/resource/pubmed/chemical/Fluoresceins,
http://linkedlifedata.com/resource/pubmed/chemical/Perylene,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylserines,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/fluorexon,
http://linkedlifedata.com/resource/pubmed/chemical/hypericin
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
462
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
295-301
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10622714-3T3 Cells,
pubmed-meshheading:10622714-Animals,
pubmed-meshheading:10622714-Apoptosis,
pubmed-meshheading:10622714-Bongkrekic Acid,
pubmed-meshheading:10622714-Caspase 3,
pubmed-meshheading:10622714-Caspases,
pubmed-meshheading:10622714-Cell Line,
pubmed-meshheading:10622714-Cell Membrane Permeability,
pubmed-meshheading:10622714-Cyclosporine,
pubmed-meshheading:10622714-Dose-Response Relationship, Radiation,
pubmed-meshheading:10622714-Enzyme Activation,
pubmed-meshheading:10622714-Fibroblasts,
pubmed-meshheading:10622714-Flow Cytometry,
pubmed-meshheading:10622714-Fluoresceins,
pubmed-meshheading:10622714-Humans,
pubmed-meshheading:10622714-Hydrogen-Ion Concentration,
pubmed-meshheading:10622714-Intracellular Membranes,
pubmed-meshheading:10622714-Membrane Potentials,
pubmed-meshheading:10622714-Mice,
pubmed-meshheading:10622714-Microscopy, Confocal,
pubmed-meshheading:10622714-Mitochondria,
pubmed-meshheading:10622714-Perylene,
pubmed-meshheading:10622714-Phosphatidylserines,
pubmed-meshheading:10622714-Photosensitivity Disorders,
pubmed-meshheading:10622714-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:10622714-Time Factors
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pubmed:year |
1999
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pubmed:articleTitle |
Over-expression of Bcl-2 does not protect cells from hypericin photo-induced mitochondrial membrane depolarization, but delays subsequent events in the apoptotic pathway.
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pubmed:affiliation |
Laboratoire de Physicochimie Biomoléculaire et Cellulaire (CNRS ESA 7033), Université P. et M. Curie, Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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