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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2000-2-2
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pubmed:databankReference |
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pubmed:abstractText |
For the biosynthesis of calendic acid a (8,11)-linoleoyl desaturase activity has been proposed. To isolate this desaturase, PCR-based cloning was used. The open reading frame of the isolated full-length cDNA is a 1131 bp sequence encoding a protein of 377 amino acids. For functional identification the cDNA was expressed in Saccharomyces cerevisiae, and formation of calendic acid was analyzed by RP-HPLC. The expression of the heterologous enzyme resulted in a significant amount of calendic acid presumably esterified within phospholipids. The results presented here identify a gene encoding a new type of (1,4)-acyl lipid desaturase.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
462
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
249-53
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10622705-Amino Acid Sequence,
pubmed-meshheading:10622705-Calendula,
pubmed-meshheading:10622705-Chromatography, High Pressure Liquid,
pubmed-meshheading:10622705-Cloning, Molecular,
pubmed-meshheading:10622705-DNA, Complementary,
pubmed-meshheading:10622705-Fatty Acid Desaturases,
pubmed-meshheading:10622705-Fatty Acids,
pubmed-meshheading:10622705-Linoleoyl-CoA Desaturase,
pubmed-meshheading:10622705-Molecular Sequence Data,
pubmed-meshheading:10622705-Phylogeny,
pubmed-meshheading:10622705-Plants, Medicinal,
pubmed-meshheading:10622705-Polymerase Chain Reaction,
pubmed-meshheading:10622705-Saccharomyces cerevisiae,
pubmed-meshheading:10622705-Sequence Homology, Amino Acid
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pubmed:year |
1999
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pubmed:articleTitle |
Isolation and characterization of a calendic acid producing (8,11)-linoleoyl desaturase.
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pubmed:affiliation |
Leibniz Institut für Pflanzenbiochemie, Weinberg, Halle, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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