Linked Life Data

10620121

Source:http://linkedlifedata.com/resource/pubmed/id/10620121

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-3-9
pubmed:abstractText
Patients with pemphigus vulgaris have circulating IgG autoantibodies against desmoglein 3, which inhibit cell-cell adhesion of keratinocytes and cause blister formation in the skin and mucous membrane. To examine cellular mechanisms underlying the autoantibody production in pemphigus vulgaris patients, we have successfully developed an enzyme-linked immunospot assay which was able to detect desmoglein 3-specific autoimmune B cells quantitatively. Circulating B cells producing anti-desmoglein 3 antibodies were detected exclusively in three patients with severe disease (1.3-2.3/105 peripheral blood mononuclear cells), but not in 10 patients with mild disease or in remission or in seven healthy individuals. When this enzyme-linked immunospot assay was combined with in vitro stimulation of peripheral blood mononuclear cells with pokeweed mitogen and recombinant-desmoglein 3, we could detect circulating desmoglein 3-specific memory B cells in nine of 14 patients (6.3-84. 0/105 peripheral blood mononuclear cells), but in none of 10 healthy individuals. We further analyzed the role of CD4 + T cells in promoting anti-desmoglein 3 antibody production. The in vitro anti-desmoglein 3 antibody production was abolished when CD4 + cells were depleted or when anti-HLA-DR or anti-HLA-DQ monoclonal antibody was added to the cultures. Our results demonstrated the quantitative detection of circulating "activated" and "memory" desmoglein 3-specific B cells and suggested the important part of HLA class II-restricted CD4 + T cells in the autoantibody production in pemphigus vulgaris. In addition, the enzyme-linked immunospot assay in combination with in vitro stimulation of B cells could be broadly applied to study mechanisms for autoantibody production in various autoimmune diseases.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-202X
pubmed:author
pubmed:issnType
Print
pubmed:volume
114
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
88-94
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10620121-Adult, pubmed-meshheading:10620121-Aged, pubmed-meshheading:10620121-Aged, 80 and over, pubmed-meshheading:10620121-Antibody Formation, pubmed-meshheading:10620121-Autoantibodies, pubmed-meshheading:10620121-B-Lymphocytes, pubmed-meshheading:10620121-Cadherins, pubmed-meshheading:10620121-Desmoglein 3, pubmed-meshheading:10620121-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:10620121-Epitopes, pubmed-meshheading:10620121-Female, pubmed-meshheading:10620121-Humans, pubmed-meshheading:10620121-Immunization, pubmed-meshheading:10620121-Immunoglobulin G, pubmed-meshheading:10620121-Immunologic Memory, pubmed-meshheading:10620121-Immunologic Techniques, pubmed-meshheading:10620121-Male, pubmed-meshheading:10620121-Middle Aged, pubmed-meshheading:10620121-Monocytes, pubmed-meshheading:10620121-Pemphigus, pubmed-meshheading:10620121-T-Lymphocytes
pubmed:year
2000
pubmed:articleTitle
Detection of antigen-specific B cells in patients with pemphigus vulgaris by enzyme-linked immunospot assay: requirement of T cell collaboration for autoantibody production.
pubmed:affiliation
Department of Dermatology, Keio University School of Medicine, Tokyo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't