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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2000-2-10
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pubmed:abstractText |
Hepatitis delta virus (HDV) replicates its circular RNA genome via a rolling circle mechanism. During this process, cis-acting ribozymes cleave adjacent upstream sequences and thereby resolve replication intermediates to unit-length RNA. The subsequent ligation of these 5'OH and 2',3'-cyclic phosphate termini to form circular RNA is an essential step in the life cycle of the virus. Here we present evidence for the involvement of a host activity in the ligation of HDV RNA. We used both HDV and hammerhead ribozymes to generate a panel of HDV and non-HDV RNA substrates that bear 5' hydroxyl and 2', 3'- cyclic phosphate termini. We found that ligation of these substrates occurred in host cells, but not in vitro or in Escherichia coli. The host-specific ligation activity was capable of joining RNA in both bimolecular and intramolecular reactions and functioned in a sequence-independent manner. We conclude that mammalian cells contain a default pathway that efficiently circularizes ribozyme processed RNAs. This pathway could be exploited in the delivery of stable antisense and decoy RNA to the nucleus.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-10376872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-1318785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-1537841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-1922054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-2261458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-2263447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-2398903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-2457170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-2492677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-2915386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-3367426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-3512545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-3856222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-6163133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-6197756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-6297798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-6796956,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-6828385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-6863293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-6934539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-7529331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-7532538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-8151758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-8183899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-8643395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-8898193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-8898194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618434-9783582
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
4
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pubmed:volume |
97
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
424-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10618434-Base Sequence,
pubmed-meshheading:10618434-Blotting, Northern,
pubmed-meshheading:10618434-Cell Line,
pubmed-meshheading:10618434-DNA-Directed RNA Polymerases,
pubmed-meshheading:10618434-Hepatitis Delta Virus,
pubmed-meshheading:10618434-Humans,
pubmed-meshheading:10618434-Molecular Sequence Data,
pubmed-meshheading:10618434-Mutation,
pubmed-meshheading:10618434-Nucleic Acid Conformation,
pubmed-meshheading:10618434-Polynucleotide Ligases,
pubmed-meshheading:10618434-RNA,
pubmed-meshheading:10618434-RNA, Catalytic,
pubmed-meshheading:10618434-RNA, Viral,
pubmed-meshheading:10618434-RNA Precursors,
pubmed-meshheading:10618434-Viral Proteins
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pubmed:year |
2000
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pubmed:articleTitle |
A host-specific function is required for ligation of a wide variety of ribozyme-processed RNAs.
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pubmed:affiliation |
Department of Molecular Biology, Raymond and Beverly Sackler Research Foundation Laboratory, Tufts University School of Medicine, Boston, MA 02111, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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