|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
2000-2-10
|
|
pubmed:abstractText |
A method for cell-cell and cell-liposome fusion at the single-cell level is described. Individual cells or liposomes were first selected and manipulated either by optical trapping or by adhesion to a micromanipulator-controlled ultramicroelectrode. Spatially selective fusion of the cell-cell or cell-liposome pair was achieved by the application of a highly focused electric field through a pair of 5-micrometer o.d. carbon-fiber ultramicroelectrodes. The ability to fuse together single cells opens new possibilities in the manipulation of the genetic and cellular makeup of individual cells in a controlled manner. In the study of cellular networks, for example, the alteration of the biochemical identity of a selected cell can have a profound effect on the behavior of the entire network. Fusion of a single liposome with a target cell allows the introduction of the liposomal content into the cell interior as well as the addition of lipids and membrane proteins onto the cell surface. This cell-liposome fusion represents an approach to the manipulation of the cytoplasmic contents and surface properties of single cells. As an example, we have introduced a membrane protein (gamma-glutamyltransferase) reconstituted in liposomes into the cell plasma membrane.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618361-10082457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618361-1577585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618361-1760502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618361-1764975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618361-2230148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618361-2460141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618361-6414845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618361-7480833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618361-7627553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618361-8350753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618361-8528399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618361-8599657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618361-8876154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618361-9039911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618361-9164157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618361-935875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618361-9469805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618361-9788951
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0027-8424
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
4
|
|
pubmed:volume |
97
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
7-11
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:10618361-Animals,
pubmed-meshheading:10618361-Cell Fusion,
pubmed-meshheading:10618361-Cell Line,
pubmed-meshheading:10618361-Cell Membrane,
pubmed-meshheading:10618361-Electromagnetic Fields,
pubmed-meshheading:10618361-Humans,
pubmed-meshheading:10618361-Liposomes,
pubmed-meshheading:10618361-Microelectrodes,
pubmed-meshheading:10618361-Microscopy, Fluorescence,
pubmed-meshheading:10618361-gamma-Glutamyltransferase
|
|
pubmed:year |
2000
|
|
pubmed:articleTitle |
Manipulating the genetic identity and biochemical surface properties of individual cells with electric-field-induced fusion.
|
|
pubmed:affiliation |
Department of Chemistry, Göteborg University, SE-412 96 Göteborg, Sweden.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
