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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2000-2-9
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pubmed:abstractText |
Streptococcus milleri NMSCC 061 was screened for antimicrobial substances and shown to produce a bacteriolytic cell wall hydrolase, termed millericin B. The enzyme was purified to homogeneity by a four-step purification procedure that consisted of ammonium sulfate precipitation followed by gel filtration, ultrafiltration, and ion-exchange chromatography. The yield following ion-exchange chromatography was 6.4%, with a greater-than-2,000-fold increase in specific activity. The molecular weight of the enzyme was 28,924 as determined by electrospray mass spectrometry. The amino acid sequences of both the N terminus of the enzyme (NH(2) SENDFSLAMVSN) and an internal fragment which was generated by cyanogen bromide cleavage (NH(2) SIQTNAPWGL) were determined by automated Edman degradation. Millericin B displayed a broad spectrum of activity against gram-positive bacteria but was not active against Bacillus subtilis W23 or Escherichia coli ATCC 486 or against the producer strain itself. N-Dinitrophenyl derivatization and hydrazine hydrolysis of free amino and free carboxyl groups liberated from peptidoglycan digested with millericin B followed by thin-layer chromatography showed millericin B to be an endopeptidase with multiple activities. It cleaves the stem peptide at the N terminus of glutamic acid as well as the N terminus of the last residue in the interpeptide cross-link of susceptible strains.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618198-1027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618198-10336488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618198-14087370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618198-14171453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618198-14317407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618198-1740784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618198-2730641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618198-2890620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618198-3547405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618198-4015073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618198-4082823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618198-4631319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618198-4632317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618198-4960294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618198-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618198-6111553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618198-6811442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618198-7068534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618198-7747966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618198-8331084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618198-8953725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618198-9168135
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0099-2240
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
66
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
23-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2000
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pubmed:articleTitle |
Purification and partial characterization of a murein hydrolase, millericin B, produced by Streptococcus milleri NMSCC 061.
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pubmed:affiliation |
School of Molecular and Cellular Biosciences, University of Natal, Pietermaritzburg, Scottsville, South Africa.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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