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rdf:type |
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lifeskim:mentions |
umls-concept:C0001613,
umls-concept:C0007776,
umls-concept:C0022655,
umls-concept:C0022702,
umls-concept:C0027882,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0035820,
umls-concept:C0039062,
umls-concept:C0392747,
umls-concept:C0443172,
umls-concept:C0458003,
umls-concept:C0542341,
umls-concept:C0678723,
umls-concept:C1709852
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pubmed:dateCreated |
2000-2-18
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pubmed:abstractText |
We examined the maturation of GABAA receptor synapses in cortical pyramidal neurons cultured from embryonic rats. The decay kinetics of GABAA receptor-mediated miniature postsynaptic currents (mPSCs) were compared with those of responses evoked by GABA in excised membrane patches. Fast perfusion of 1 or 10 mM GABA on membrane patches evoked currents with different desensitizing time courses in young and old neurons. For neurons older than 4 days in vitro (DIV), GABAA currents had a fast component of desensitization (median approximately 3 ms) seldom seen in patches from younger neurons. In contrast, mPSCs exhibited a substantial fast component of decay at 2-4 DIV that became more prominent with further development although the median value of its time constant remained unchanged. The selective alpha3 subunit positive modulator SB-205384 had no effect on mPSCs at any time in vitro but potentiated extrasynaptic activity. This suggests that synapse maturation does not proceed by a gradual exchange of early embryonic GABAA receptor subforms for adult forms. At all ages, the kinetic properties of mPSCs were heterogeneous. This heterogeneity extended to the level of mPSCs from single neurons and may be a normal aspect of synaptic functioning. These results suggest that inhibitory synapses in developing neurons are capable of selectively capturing GABAA receptors having fast desensitization kinetics. This functional preference probably reflects the developmental turning point from an inwardly looking trophic capacity of embryonic GABAA receptors to a role concerned with information processing.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-1312131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-1322978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-1331359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-1357584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-1672071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-1694446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-7509352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-7542462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-7596410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-7722619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-7854418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-8035217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-8057250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-8145733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-8519594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-8557845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-8558466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-8568677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-8613808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-8642407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-8782110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-8815919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-8845153,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-8847645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-8876241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-8930242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-8951718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-9003050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-9133407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-9161987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-9169528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-9185549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-9257911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-9295368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-9315884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-9331359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-9334415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-9364667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-9464994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-9502804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-9503333,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-9559912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-9819245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618148-9831714
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
522 Pt 1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3-17
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10618148-Aminopyridines,
pubmed-meshheading:10618148-Animals,
pubmed-meshheading:10618148-Cell Aging,
pubmed-meshheading:10618148-Cells, Cultured,
pubmed-meshheading:10618148-Cerebral Cortex,
pubmed-meshheading:10618148-Electric Conductivity,
pubmed-meshheading:10618148-Electrophysiology,
pubmed-meshheading:10618148-Embryo, Mammalian,
pubmed-meshheading:10618148-GABA Modulators,
pubmed-meshheading:10618148-Kinetics,
pubmed-meshheading:10618148-Neurons,
pubmed-meshheading:10618148-Rats,
pubmed-meshheading:10618148-Rats, Sprague-Dawley,
pubmed-meshheading:10618148-Receptors, GABA-A,
pubmed-meshheading:10618148-Synapses,
pubmed-meshheading:10618148-Thiophenes
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pubmed:year |
2000
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