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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2000-2-17
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pubmed:abstractText |
A broad-range bacterial PCR targeting rRNA genes (rDNAs) was used to directly analyze 536 clinical samples obtained from 459 hospitalized patients during a 4-year study period. The molecular diagnosis based on DNA sequencing of the PCR product was compared to that obtained by bacterial culture. The bacteriological diagnosis was concordant for 447 (83%) specimens. Broad-range rDNA PCR was the only method that yielded an etiologic diagnosis for 11 (2.4%) of 459 patients. Compared to culture and clinical assessment, the sensitivity of the PCR method combined with sequencing was 74.2%, and the specificity was between 98.7 and 99.6%. At present, the described molecular approach proved superior to bacterial culture in two clinical situations: infections caused by bacteria with unusual growth requirements and specimens taken during antimicrobial treatment of the patient.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-10555315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-1371061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-1377787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-1380289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-1599522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-1714530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-1987160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-2233945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-2413450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-2439888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-3162770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-3431465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-3911275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-6771870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-7535888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-8085261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-8590690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-8645836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-8688393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-9195088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-9350723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-9665992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-9687486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-9707307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-9733676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-9817860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-9847132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-9847133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-9847171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618059-9894625
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0095-1137
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pubmed:author |
pubmed-author:AlanenAA,
pubmed-author:EerolaEE,
pubmed-author:JalavaJJ,
pubmed-author:KotilainenEE,
pubmed-author:KotilainenPP,
pubmed-author:MeurmanOO,
pubmed-author:NikkariSS,
pubmed-author:Rantakokko-JalavaKK,
pubmed-author:RuuskanenOO,
pubmed-author:SkurnikMM,
pubmed-author:ToivanenPP
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pubmed:issnType |
Print
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pubmed:volume |
38
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
32-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10618059-Anti-Bacterial Agents,
pubmed-meshheading:10618059-Bacteria,
pubmed-meshheading:10618059-Bacterial Infections,
pubmed-meshheading:10618059-Bacteriological Techniques,
pubmed-meshheading:10618059-Databases, Factual,
pubmed-meshheading:10618059-Finland,
pubmed-meshheading:10618059-Humans,
pubmed-meshheading:10618059-Polymerase Chain Reaction,
pubmed-meshheading:10618059-RNA, Ribosomal,
pubmed-meshheading:10618059-RNA, Ribosomal, 16S,
pubmed-meshheading:10618059-RNA, Ribosomal, 23S,
pubmed-meshheading:10618059-Reproducibility of Results,
pubmed-meshheading:10618059-Sequence Analysis, DNA
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pubmed:year |
2000
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pubmed:articleTitle |
Direct amplification of rRNA genes in diagnosis of bacterial infections.
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pubmed:affiliation |
Department of Medical Microbiology, University of Turku, Turku, Finland. kaisu.rantakokko@utu.fi
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pubmed:publicationType |
Journal Article,
Comparative Study
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