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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2000-1-31
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pubmed:abstractText |
Reactive oxygen species have been implicated in the pathogenesis of atherosclerosis and hypertension, in part by promoting vascular smooth muscle cell (VSMC) growth. We have previously shown that LY83583, a generator of O-(2), activated extracellular signal-regulated kinases (ERK1/2) with early (10 min) and late (2 h) peaks and stimulated VSMC growth. To investigate whether secreted oxidative stress-induced factors (termed SOXF) from VSMC were responsible for late ERK1/2 activation in response to LY83583, we purified putative SOXF proteins from conditioned medium (2 h of LY83583 exposure) by sequential chromatography based on activation of ERK1/2. Proteins identified by capillary chromatography, electrospray ionization tandem mass spectrometry, and data base searching included heat shock protein 90-alpha (HSP90-alpha) and cyclophilin B. Western blot analysis of conditioned medium showed specific secretion of HSP90-alpha but not HSP90-beta. Immunodepletion of HSP90-alpha from conditioned medium significantly inhibited conditioned medium-induced ERK1/2 activation. Human recombinant HSP90-alpha reproduced the effect of conditioned medium on ERK1/2 activation. These results show that brief oxidative stress causes sustained release of protein factors from VSMC that can stimulate ERK1/2. These factors may be important mediators for the effects of reactive oxygen species on vascular function.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1,2-Dihydroxybenzene-3,5-Disulfonic...,
http://linkedlifedata.com/resource/pubmed/chemical/6-anilino-5,8-quinolinedione,
http://linkedlifedata.com/resource/pubmed/chemical/Aminoquinolines,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclophilins,
http://linkedlifedata.com/resource/pubmed/chemical/HSP90 Heat-Shock Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Immunophilins,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Peptidylprolyl Isomerase,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/Trypsin,
http://linkedlifedata.com/resource/pubmed/chemical/cyclophilin B
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
7
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pubmed:volume |
275
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
189-96
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10617604-1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt,
pubmed-meshheading:10617604-Aminoquinolines,
pubmed-meshheading:10617604-Animals,
pubmed-meshheading:10617604-Autocrine Communication,
pubmed-meshheading:10617604-Culture Media, Conditioned,
pubmed-meshheading:10617604-Cyclophilins,
pubmed-meshheading:10617604-HSP90 Heat-Shock Proteins,
pubmed-meshheading:10617604-Immunophilins,
pubmed-meshheading:10617604-Male,
pubmed-meshheading:10617604-Mitogen-Activated Protein Kinase 1,
pubmed-meshheading:10617604-Mitogen-Activated Protein Kinase 3,
pubmed-meshheading:10617604-Mitogen-Activated Protein Kinases,
pubmed-meshheading:10617604-Muscle, Smooth, Vascular,
pubmed-meshheading:10617604-Oxidative Stress,
pubmed-meshheading:10617604-Peptidylprolyl Isomerase,
pubmed-meshheading:10617604-Rats,
pubmed-meshheading:10617604-Rats, Sprague-Dawley,
pubmed-meshheading:10617604-Reactive Oxygen Species,
pubmed-meshheading:10617604-Trypsin
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pubmed:year |
2000
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pubmed:articleTitle |
Purification and identification of secreted oxidative stress-induced factors from vascular smooth muscle cells.
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pubmed:affiliation |
Center for Cardiovascular Research, University of Rochester Medical Center, Rochester, New York 14642, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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