Source:http://linkedlifedata.com/resource/pubmed/id/10616526
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
2000-1-13
|
pubmed:abstractText |
We report the successful fusion of human choriocarcinoma cells with normal human trophoblast cells to a choriocarcinoma/trophoblast hybrid. The hybrid cells ACH1P were derived from fusion of primary male trophoblast cells with the HGPRT-defective choriocarcinoma cell line AC1-1. The karyotypes of the parental choriocarcinoma cell line JEG-3, its HGPRT-defective mutant clones AC1-1, AC1-5, and AC1-9, and the choriocarcinoma/trophoblast hybrid ACH1P are presented, together with a detailed characterization of the AC1-specific chromosomal marker add(X)(q26) using conventional cytogenetic banding techniques and multiplex-fluorescence in situ hybridization (M-FISH). To our knowledge, this is the first report of a stably proliferating human cell hybrid of trophoblastic origin, providing a unique cell culture model to study trophoblast-related invasion and its underlying genetic mechanisms.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jan
|
pubmed:issn |
0165-4608
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
1
|
pubmed:volume |
116
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
16-22
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:10616526-Cell Fusion,
pubmed-meshheading:10616526-Choriocarcinoma,
pubmed-meshheading:10616526-Chromosome Banding,
pubmed-meshheading:10616526-DNA Fingerprinting,
pubmed-meshheading:10616526-Female,
pubmed-meshheading:10616526-Humans,
pubmed-meshheading:10616526-Hybrid Cells,
pubmed-meshheading:10616526-In Situ Hybridization, Fluorescence,
pubmed-meshheading:10616526-Karyotyping,
pubmed-meshheading:10616526-Pregnancy,
pubmed-meshheading:10616526-Trophoblasts,
pubmed-meshheading:10616526-Tumor Cells, Cultured,
pubmed-meshheading:10616526-Uterine Neoplasms
|
pubmed:year |
2000
|
pubmed:articleTitle |
Cytogenetic and DNA-fingerprint characterization of choriocarcinoma cell lines and a trophoblast/choriocarcinoma cell hybrid.
|
pubmed:affiliation |
Department of Anatomy, Technical University of Aachen, Germany.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|