Linked Life Data

10615682

Source:http://linkedlifedata.com/resource/pubmed/id/10615682

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2000-1-19
pubmed:abstractText
The strain BAFC 2336 of Curvularia pallescens is a hyphomycete isolated from internal fungi present in leaves and stems of Baccharis coridifolia. Three compounds designated B, D1 and D2 which inhibited the replication of vesicular stomatitis virus (VSV) and herpes simplex virus type 1 (HSV-1) in tissue cultures were isolated from fluid cultures of Curvularia pallescens. The purification procedure of the compounds consisted first in an organic solvent extraction followed by chromatography through a silica gel column. Fractions eluted from the column with antiviral activity were pooled and then subjected to thin layer chromatography (TLC) in silica gel plates. Three isolated bands were recognized with Rf of 0.63, 0.36 y 0.33 for B, D1 and D2, respectively. The chromatographic characteristics of the isolated metabolites were determined by TLC and HPLC and their chemical structure by means of spectroscopic methods. Analysis of the data obtained indicated that compound D2 (MW: 280 Da) is identical with Brefeldin A a macrolide with antiviral activity isolated from other fungi but not reported to be present in Curvularia pallescens. Compound D1 is similar in structure to compound D2; however, the low amount obtained after purification unabled us to obtain complete structural characterization. Compound B (MW: 332 Da) has an aromatic ring and a chemical structure related to curvularins, a generic name for certain metabolites from Curvularia. This compound appears to be a novel compound with antiviral potency similar to Brefeldin A, but less toxic.
pubmed:language
por
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0325-7541
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
193-200
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
[Antiviral activity metabolites isolated from Curvularia pallescens].
pubmed:affiliation
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't