Linked Life Data

10614647

Source:http://linkedlifedata.com/resource/pubmed/id/10614647

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-1-13
pubmed:abstractText
Recently, we cloned a messenger RNA (mRNA) predominantly expressed in chondrocytes from a human chondrosarcoma-derived chondrocytic cell line, HCS-2/8, by differential display PCR and found that its gene, named hcs24, was identical with that of connective tissue growth factor (CTGF). Here we investigated CTGF/Hcs24 function in the chondrocytic cell line HCS-2/8 and rabbit growth cartilage (RGC) cells. HCS-2/8 cells transfected with recombinant adenoviruses that generate CTGF/Hcs24 sense RNA (mRNA) proliferated more rapidly than HCS-2/8 cells transfected with control adenoviruses. HCS-2/8 cells transfected with recombinant adenoviruses that generate CTGF/Hcs24 sense RNA expressed more mRNA of aggrecan and type X collagen than the control cells. To elucidate the direct action of CTGF/Hcs24 on the cells, we transfected HeLa cells with CTGF/Hcs24 expression vectors, obtained stable transfectants, and purified recombinant CTGF/Hcs24 protein from conditioned medium of the transfectants. The recombinant CTGF/Hcs24 effectively promoted the proliferation of HCS-2/8 cells and RGC cells in a dose-dependent manner and also dose dependently increased proteoglycan synthesis in these cells. In addition, these stimulatory effects of CTGF/Hcs24 were neutralized by the addition of anti-CTGF antibodies. Furthermore, the recombinant CTGF/Hcs24 effectively increased alkaline phosphatase activity in RGC cells in culture. Moreover, RT-PCR analysis revealed that the recombinant CTGF/Hcs24 stimulated gene expression of aggrecan and collagen types II and X in RGC cells in culture. These results indicate that CTGF/Hcs24 directly promotes the proliferation and differentiation of chondrocytes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
141
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
264-73
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10614647-Adenoviridae, pubmed-meshheading:10614647-Animals, pubmed-meshheading:10614647-Blotting, Western, pubmed-meshheading:10614647-Cartilage, pubmed-meshheading:10614647-Cell Differentiation, pubmed-meshheading:10614647-Cell Division, pubmed-meshheading:10614647-Cells, Cultured, pubmed-meshheading:10614647-Chondrocytes, pubmed-meshheading:10614647-Collagen, pubmed-meshheading:10614647-Connective Tissue Growth Factor, pubmed-meshheading:10614647-DNA, Neoplasm, pubmed-meshheading:10614647-Growth Substances, pubmed-meshheading:10614647-Humans, pubmed-meshheading:10614647-Immediate-Early Proteins, pubmed-meshheading:10614647-Intercellular Signaling Peptides and Proteins, pubmed-meshheading:10614647-Proteoglycans, pubmed-meshheading:10614647-RNA, Messenger, pubmed-meshheading:10614647-Rabbits, pubmed-meshheading:10614647-Recombinant Proteins, pubmed-meshheading:10614647-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:10614647-Transfection
pubmed:year
2000
pubmed:articleTitle
Effects of CTGF/Hcs24, a product of a hypertrophic chondrocyte-specific gene, on the proliferation and differentiation of chondrocytes in culture.
pubmed:affiliation
Department of Biochemistry and Molecular Dentistry, Biodental Research Center, Okayama University Dental School, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't