|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0008633,
umls-concept:C0017337,
umls-concept:C0038952,
umls-concept:C0080194,
umls-concept:C0086418,
umls-concept:C0120285,
umls-concept:C0678170,
umls-concept:C0679083,
umls-concept:C0699819,
umls-concept:C1158478,
umls-concept:C1705422,
umls-concept:C1706089
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
2000-3-20
|
|
pubmed:abstractText |
An integration vector was constructed to allow introduction of the gfp gene into the chromosomes of Gram-positive bacteria. Integration depends on homologous recombination between a short 458-nt sequence of the tet(M) gene in the vector and a copy of Tn916 in the host chromosome. Strains of Lactococcus lactis IL1403, Enterococcus faecalis JH2-SS, and Streptococcus gordonii DL1 stably marked with single chromosomal copies of the gfp were readily visualised by epifluorescence microscopy. The marked L. lactis strain survived poorly in a continuous culture system inoculated with human faecal flora, while the laboratory E. faecalis strain was lost at approximately the dilution rate of the fermenter.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0378-1097
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
182
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
23-7
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:10612725-Bacteria,
pubmed-meshheading:10612725-Chromosomes, Bacterial,
pubmed-meshheading:10612725-Colony Count, Microbial,
pubmed-meshheading:10612725-DNA Transposable Elements,
pubmed-meshheading:10612725-Digestive System,
pubmed-meshheading:10612725-Enterococcus faecalis,
pubmed-meshheading:10612725-Feces,
pubmed-meshheading:10612725-Fermentation,
pubmed-meshheading:10612725-Genetic Markers,
pubmed-meshheading:10612725-Genetic Vectors,
pubmed-meshheading:10612725-Gram-Positive Bacteria,
pubmed-meshheading:10612725-Green Fluorescent Proteins,
pubmed-meshheading:10612725-Humans,
pubmed-meshheading:10612725-Lactic Acid,
pubmed-meshheading:10612725-Lactococcus lactis,
pubmed-meshheading:10612725-Luminescent Proteins,
pubmed-meshheading:10612725-Microscopy, Fluorescence,
pubmed-meshheading:10612725-Recombination, Genetic,
pubmed-meshheading:10612725-Streptococcus,
pubmed-meshheading:10612725-Tetracycline Resistance,
pubmed-meshheading:10612725-Transformation, Bacterial
|
|
pubmed:year |
2000
|
|
pubmed:articleTitle |
Chromosomal integration of the green fluorescent protein gene in lactic acid bacteria and the survival of marked strains in human gut simulations.
|
|
pubmed:affiliation |
Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen, UK. kps@rri.sari.ac.uk
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
