Linked Life Data

10612421

Source:http://linkedlifedata.com/resource/pubmed/id/10612421

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2000-1-13
pubmed:abstractText
In rat astroglial cells, four G(omicronalpha) transcripts were found: G(omicron2alpha) mRNA (5.7 kb) and three G(omicron1alpha) mRNAs (4.0, 3.0 and 2.3 kb). However, G(omicron2alpha) but little G(omicronalpha1) proteins were present in membrane-enriched fractions. Culturing astroglial cells with forskolin (10 microM) or isoproterenol (10 microM) a beta-adrenergic agonist increased transiently in a time-dependent manner the levels of G(omicronalpha) proteins. The degradation rate of G(omicronalpha) proteins was slightly decreased by the cAMP treatment. In parallel, forskolin (10 microM) treatment increased transiently the amounts of both G(omicron1alpha) and G(omicron2alpha) mRNAs. The relative transcription rate of G(omicronalpha) gene was increased by 1.7-fold in forskolin-treated cells whereas the half-lives of G(omicron1alpha) and G(omicron2alpha) mRNAs were not significantly changed. These results suggest that cAMP regulates the transcription rate of G(omicronalpha) gene and this is compatible with the existence of a cAMP responsive element in the promoter of the G(omicronalpha) gene.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0303-7207
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
156
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
35-43
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Cyclic AMP regulates G(omicronalpha) protein and mRNA levels by modulating the transcriptional rate of G(omicronalpha) gene.
pubmed:affiliation
Unité de Recherche, Transduction Hormonale et Régulation Cellulaire, U 486 INSERM, Faculté de Pharmacie 5, Châtenay-Malabry, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't