Source:http://linkedlifedata.com/resource/pubmed/id/10611889
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
369
|
| pubmed:dateCreated |
2000-1-14
|
| pubmed:abstractText |
Recombinant adenovirus mediated human bone morphogenetic protein-12 gene transfer induced tendon and cartilage-like tissue formation in vivo. The recombinant adenovirus with the human bone morphogenetic protein-12 gene was constructed, and mature human bone morphogenetic protein-12 expression mediated by adenovirus gene transfer was detected by specific antibody. Unlike bone morphogenetic protein-2 gene transfer, bone morphogenetic protein-12 gene transferred mesenchymal progenitor cell line C3H 10T1/2 showed no change of alkaline phosphatase activity, which is the mark of cell differentiation into osteoblastic phenotype. Injection of bone morphogenetic protein-12 gene transferred C3H 10T1/2 cells into nude mice thigh muscles induced tendon and cartilage-like tissue formation. The results indicate bone morphogenetic protein-12 has different effects on mesenchymal progenitor cell differentiation, and it may influence the cell differentiation into a nonosteoblast lineage.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0009-921X
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
333-9
|
| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10611889-Adenoviridae,
pubmed-meshheading:10611889-Alkaline Phosphatase,
pubmed-meshheading:10611889-Animals,
pubmed-meshheading:10611889-Blotting, Western,
pubmed-meshheading:10611889-Bone Morphogenetic Proteins,
pubmed-meshheading:10611889-Cartilage,
pubmed-meshheading:10611889-Cell Differentiation,
pubmed-meshheading:10611889-Cells, Cultured,
pubmed-meshheading:10611889-Gene Expression Regulation,
pubmed-meshheading:10611889-Gene Transfer Techniques,
pubmed-meshheading:10611889-Hindlimb,
pubmed-meshheading:10611889-Humans,
pubmed-meshheading:10611889-Mesoderm,
pubmed-meshheading:10611889-Mice,
pubmed-meshheading:10611889-Mice, Inbred C3H,
pubmed-meshheading:10611889-Recombination, Genetic,
pubmed-meshheading:10611889-Stem Cells,
pubmed-meshheading:10611889-Tendons
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Effect of bone morphogenetic protein-12 gene transfer on mesenchymal progenitor cells.
|
| pubmed:affiliation |
Department of Orthopaedic Surgery, Washington University School of Medicine, St Louis, MO 63110, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|