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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
26
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pubmed:dateCreated |
2000-1-27
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pubmed:databankReference |
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pubmed:abstractText |
The ubiquitin-like protein RUB1 is conjugated to target proteins by a mechanism similar to that of ubiquitin conjugation. Genetic studies in Arabidopsis thaliana have implicated the RUB-conjugation pathway in auxin response. The first step in the pathway is RUB activation by a bipartite enzyme composed of the AXR1 and ECR1 proteins. Ubiquitin activation is an ATP-dependent process that involves the formation of an AMP-ubiquitin intermediate. Here we show that RUB activation by AXR1-ECR1 also involves formation of an AMP-RUB intermediate and that this reaction is catalyzed by the ECR1 subunit alone. In addition, we identified an Arabidopsis protein called RCE1 that is a likely RUB-conjugating enzyme. RCE1 works together with AXR1-ECR1 to promote formation of a stable RUB conjugate with the Arabidopsis cullin AtCUL1 in vitro. Using a tagged version of RUB1, we show that this modification occurs in vivo. Because AtCUL1 is a component of the ubiquitin protein ligase SCF(TIR1), a complex that also functions in auxin response, we propose that RUB modification of AtCUL1 is important for auxin response.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10611386-10066623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10611386-10092517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10611386-10318914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10611386-10398681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10611386-1634524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10611386-2193438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10611386-6277905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10611386-6313038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10611386-8155884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10611386-8978815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10611386-9312010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10611386-9409544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10611386-9427741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10611386-9436980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10611386-9531529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10611386-9531531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10611386-9545234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10611386-9624055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10611386-9635407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10611386-9694792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10611386-9759494
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AXR1 protein, Arabidopsis,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Monophosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Arabidopsis Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/ECR1 protein, Arabidopsis,
http://linkedlifedata.com/resource/pubmed/chemical/Esters,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Ligases,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RUB1 protein, Arabidopsis,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfhydryl Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin-Conjugating Enzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin-Protein Ligases,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitins
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
21
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pubmed:volume |
96
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
15342-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10611386-Adenosine Monophosphate,
pubmed-meshheading:10611386-Amino Acid Sequence,
pubmed-meshheading:10611386-Arabidopsis,
pubmed-meshheading:10611386-Arabidopsis Proteins,
pubmed-meshheading:10611386-Conserved Sequence,
pubmed-meshheading:10611386-Esters,
pubmed-meshheading:10611386-Growth Substances,
pubmed-meshheading:10611386-Ligases,
pubmed-meshheading:10611386-Molecular Sequence Data,
pubmed-meshheading:10611386-Plant Proteins,
pubmed-meshheading:10611386-Protein Processing, Post-Translational,
pubmed-meshheading:10611386-Sequence Homology, Amino Acid,
pubmed-meshheading:10611386-Sulfhydryl Compounds,
pubmed-meshheading:10611386-Ubiquitin-Conjugating Enzymes,
pubmed-meshheading:10611386-Ubiquitin-Protein Ligases,
pubmed-meshheading:10611386-Ubiquitins
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pubmed:year |
1999
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pubmed:articleTitle |
The Arabidopsis cullin AtCUL1 is modified by the ubiquitin-related protein RUB1.
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pubmed:affiliation |
Institute for Cellular and Molecular Biology, Section of Molecular, Biology, University of Texas at Austin, Austin, TX 78712, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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