Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
26
pubmed:dateCreated
2000-1-27
pubmed:abstractText
Site-directed mutagenesis and combinatorial libraries are powerful tools for providing information about the relationship between protein sequence and structure. Here we report two extensions that expand the utility of combinatorial mutagenesis for the quantitative assessment of hypotheses about the determinants of protein structure. First, we show that resin-splitting technology, which allows the construction of arbitrarily complex libraries of degenerate oligonucleotides, can be used to construct more complex protein libraries for hypothesis testing than can be constructed from oligonucleotides limited to degenerate codons. Second, using eglin c as a model protein, we show that regression analysis of activity scores from library data can be used to assess the relative contributions to the specific activity of the amino acids that were varied in the library. The regression parameters derived from the analysis of a 455-member sample from a library wherein four solvent-exposed sites in an alpha-helix can contain any of nine different amino acids are highly correlated (P < 0.0001, R(2) = 0. 97) to the relative helix propensities for those amino acids, as estimated by a variety of biophysical and computational techniques.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-10193194, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-10500156, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-10639110, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-1304915, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-1404368, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-1404369, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-1460281, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-1610812, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-1653604, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-1853201, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-1931967, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-2038061, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-2147779, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-2207109, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-2237416, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-2524006, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-2837824, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-3196687, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-3381086, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-3388019, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-3561498, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-681366, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-7490748, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-7535612, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-7626743, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-7664054, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-7670375, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-7731949, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-7844817, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-7937708, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-7937968, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-8061613, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-8176729, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-8259512, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-8259514, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-8278384, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-8332196, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-8347570, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-8380714, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-8503008, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-8612075, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-8643620, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-8732753, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-8880933, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-8900163, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-9096306, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-9334745, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-9416601, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-9514257, http://linkedlifedata.com/resource/pubmed/commentcorrection/10611303-9750153
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
21
pubmed:volume
96
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
14860-5
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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