Source:http://linkedlifedata.com/resource/pubmed/id/10607914
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2000-3-15
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| pubmed:abstractText |
The development of the synovial joint cavity between the cartilage anlagen of the long bones is thought to be mediated by differential matrix synthesis at the developing articular surfaces. In addition, many studies have shown that removal of movement-induced mechanical stimuli from developing diarthrodial joints prevents cavity formation or produces a secondary fusion of previously cavitated joints. Herein, we describe an inductive influence of mechanical strain on hyaluronan metabolism and the expression of hyaluronan-binding proteins in cultured cells isolated from the articular surface of the distal tibial condyles of 18-day chick embryos. The effect of 10 min of mechanical strain on hyaluronan release into culture media, intracellular uridine diphospho-glucose dehydrogenase activity (an enzyme required for hyaluronan saccharide precursor production), cell surface hyaluronan-binding protein expression and HA synthase mRNA expression were analysed up to 24 h later. Six hours after the application of strain, there was a significant increase in the accumulation of hyaluronan released into tissue culture media by strained fibrocartilage cells compared with controls, an effect still detectable after 24 h. Strained cells also showed increased activity for uridine diphospho-glucose dehydrogenase and expressed higher levels of the hyaluronan-binding protein CD44 at 24 h. In addition, at 24 h mRNA for HA synthase 2 was expressed in all samples whereas mRNA for HA synthase 3 was only expressed in strained cells. These results further highlight the role for movement-induced stimuli in differential extracellular matrix metabolism during joint development and also show that strain may facilitate differential HA synthase gene expression.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD44,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Glucuronosyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Glycosyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/HAS1 protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/Hyaluronic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Transferases,
http://linkedlifedata.com/resource/pubmed/chemical/Uridine Diphosphate Glucose...,
http://linkedlifedata.com/resource/pubmed/chemical/Xenopus Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/hyaluronan synthase
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| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
0945-053X
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
18
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
523-32
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10607914-Animals,
pubmed-meshheading:10607914-Antigens, CD44,
pubmed-meshheading:10607914-Base Sequence,
pubmed-meshheading:10607914-Cartilage, Articular,
pubmed-meshheading:10607914-Cells, Cultured,
pubmed-meshheading:10607914-Chick Embryo,
pubmed-meshheading:10607914-DNA Primers,
pubmed-meshheading:10607914-Extracellular Matrix,
pubmed-meshheading:10607914-Gene Expression,
pubmed-meshheading:10607914-Glucuronosyltransferase,
pubmed-meshheading:10607914-Glycosyltransferases,
pubmed-meshheading:10607914-Hyaluronic Acid,
pubmed-meshheading:10607914-Immunohistochemistry,
pubmed-meshheading:10607914-Membrane Proteins,
pubmed-meshheading:10607914-RNA, Messenger,
pubmed-meshheading:10607914-Stress, Mechanical,
pubmed-meshheading:10607914-Transferases,
pubmed-meshheading:10607914-Uridine Diphosphate Glucose Dehydrogenase,
pubmed-meshheading:10607914-Xenopus Proteins
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| pubmed:year |
1999
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| pubmed:articleTitle |
The effect of mechanical strain on hyaluronan metabolism in embryonic fibrocartilage cells.
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| pubmed:affiliation |
Cardiff School of Biosciences, Cardiff University, P.O. Box 911 Museum Avenue, Cardiff, UK.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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