Source:http://linkedlifedata.com/resource/pubmed/id/10607828
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2000-2-28
|
| pubmed:abstractText |
Using combined immunofluorescence and fluorescence in situ hybridization (FISH) analysis we have extensively characterized the proteins associating with two different homologue human neocentromeres at interphase and prometaphase/metaphase, and compared these directly with those found with normal human centromeres. Antisera to CENP-A, CENP-B, CENP-C, CENP-E, CENP-F, INCENP, CLIP-170, dynein, dynactin subunits p150 (Glued) and Arp1, MCAK, Tsg24, p55CDC, HZW10, HBUB1, HBUBR1, BUB3, MAD2, ERK1, 3F3/2, topoisomerase II and a murine HP1 homologue, M31, were used in immuno-fluorescence experiments in conjunction with FISH employing specific DNA probes to clearly identify neocentromeric DNA. We found that except for the total absence of CENP-B binding, neocentromeres are indistinguishable from their alpha satellite-containing counterparts in terms of protein composition and distribution. This suggests that the DNA base of a potential centromeric locus is of minimal importance in determining the overall structure of a functional kinetochore and that, once seeded, the events leading to functional kinetochore formation occur independently of primary DNA sequence.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0964-6906
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
22
|
| pubmed:volume |
9
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
175-85
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| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:10607828-Anaphase,
pubmed-meshheading:10607828-Animals,
pubmed-meshheading:10607828-CHO Cells,
pubmed-meshheading:10607828-Cell Cycle,
pubmed-meshheading:10607828-Cell Line, Transformed,
pubmed-meshheading:10607828-Centromere,
pubmed-meshheading:10607828-Chromatin,
pubmed-meshheading:10607828-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:10607828-Chromosomes, Human, Pair 10,
pubmed-meshheading:10607828-Chromosomes, Human, Pair 20,
pubmed-meshheading:10607828-Cricetinae,
pubmed-meshheading:10607828-Humans,
pubmed-meshheading:10607828-Immune Sera,
pubmed-meshheading:10607828-Kinetochores,
pubmed-meshheading:10607828-Metaphase,
pubmed-meshheading:10607828-Microtubule-Associated Proteins,
pubmed-meshheading:10607828-Protein Binding,
pubmed-meshheading:10607828-Tumor Cells, Cultured
|
| pubmed:year |
2000
|
| pubmed:articleTitle |
Human centromeres and neocentromeres show identical distribution patterns of >20 functionally important kinetochore-associated proteins.
|
| pubmed:affiliation |
The Murdoch Institute, Royal Children's Hospital, Flemington Road, Parkville 3052, Australia.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|