Source:http://linkedlifedata.com/resource/pubmed/id/10605013
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2000-1-19
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| pubmed:abstractText |
Dissecting the mechanisms of T cell-mediated immunity requires the identification of functional characteristics and surface markers that distinguish between activated and memory T lymphocytes. In this study, we compared the rates of cytokine production by virus-specific primary and memory CD8+ T cells directly ex vivo. Ag-specific IFN-gamma and TNF-alpha production by both primary and long-term memory T cells was observed in </=60 min after peptide stimulation. Although the on-rate kinetics of cytokine production were nearly identical, activated T cells produced more IFN-gamma, but less TNF-alpha, than memory T cells. Ag-specific cytokine synthesis was not a constitutive process and terminated immediately following disruption of contact with peptide-coated cells, demonstrating that continuous antigenic stimulation was required by both T cell populations to maintain steady-state cytokine production. Upon re-exposure to Ag, activated T cells resumed cytokine production whereas only a subpopulation of memory T cells reinitiated cytokine synthesis. Analysis of cytokine profiles and levels of CD8, LFA-1, and CTLA-4 together revealed a pattern of expression that clearly distinguished in vivo-activated T cells from memory T cells. Surprisingly, CTLA-4 expression was highest at the early stages of the immune response but fell to background levels soon after viral clearance. This study is the first to show that memory T cells have the same Ag-specific on/off regulation of cytokine production as activated T cells and demonstrates that memory T cells can be clearly discriminated from activated T cells directly ex vivo by their cytokine profiles and the differential expression of three well-characterized T cell markers.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD8,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/CTLA-4 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Ctla4 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes, T-Lymphocyte,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoconjugates,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphocyte Function-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/abatacept
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
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| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
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| pubmed:volume |
164
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
208-16
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:10605013-Animals,
pubmed-meshheading:10605013-Antigens, CD,
pubmed-meshheading:10605013-Antigens, CD8,
pubmed-meshheading:10605013-Antigens, Differentiation,
pubmed-meshheading:10605013-Antigens, Viral,
pubmed-meshheading:10605013-Biological Markers,
pubmed-meshheading:10605013-CD8-Positive T-Lymphocytes,
pubmed-meshheading:10605013-CTLA-4 Antigen,
pubmed-meshheading:10605013-Clone Cells,
pubmed-meshheading:10605013-Cytokines,
pubmed-meshheading:10605013-Epitopes, T-Lymphocyte,
pubmed-meshheading:10605013-Immunoconjugates,
pubmed-meshheading:10605013-Immunologic Memory,
pubmed-meshheading:10605013-Immunophenotyping,
pubmed-meshheading:10605013-Lymphocyte Activation,
pubmed-meshheading:10605013-Lymphocyte Function-Associated Antigen-1,
pubmed-meshheading:10605013-Lymphocytic choriomeningitis virus,
pubmed-meshheading:10605013-Mice,
pubmed-meshheading:10605013-Mice, Inbred BALB C,
pubmed-meshheading:10605013-T-Lymphocyte Subsets
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| pubmed:year |
2000
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| pubmed:articleTitle |
Activated and memory CD8+ T cells can be distinguished by their cytokine profiles and phenotypic markers.
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| pubmed:affiliation |
Department of Neuropharmacology, The Scripps Research Institute, La Jolla, CA 92037, USA. mslifka@scripps.edu
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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