Source:http://linkedlifedata.com/resource/pubmed/id/10604997
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2000-1-19
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| pubmed:abstractText |
CD8+ T cells down-regulate a variety of immune responses. For example, porcine and human insulin do not stimulate Abs in C57BL/6 mice because CD8+ T cells inhibit CD4+ helper T cells. By contrast, bovine insulin induces Ab in C57BL/6 mice, and removal of CD8+ T cells does not alter this response. This raises the question of whether porcine, but not bovine, insulin activates CD8+ T cells or whether both insulins activate CD8+ T cells but CD4+ helper T cells are differentially inhibited by them. In this study, we show that insulin-specific CD8+ CTL can be cultured from C57BL/6 mice primed with either bovine or human insulin in CFA. Thus, exogenous Ags, besides OVA, induce CD8+ CTL when administered in an adjuvant, suggesting this is a typical response. These CTL are H-2Kb restricted and produce IL-5, IL-10, IFN-gamma, and small amounts of IL-4, which is distinct from IFN-gamma and TNF-alpha that are typically secreted by virus-specific CTL. Moreover, the CTL primed with either bovine or human insulin recognize an A-chain peptide that is identical to the mouse insulin sequence. That foreign proteins, which are closely related to self-proteins, activated autoreactive, CD8+ T cells in vivo is a novel finding. It raises the possibility that self-reactive CTL may be activated by cross-reacting Ags and once activated they might participate in autoimmunity. These results also suggest that down-regulation of insulin-specific responses by autoreactive CD8+ T cells is most likely due to the differential sensitivity of bovine and human insulin-specific CD4+ T cells.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD8,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes, T-Lymphocyte,
http://linkedlifedata.com/resource/pubmed/chemical/H-2 Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
164
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
86-92
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:10604997-Amino Acid Sequence,
pubmed-meshheading:10604997-Animals,
pubmed-meshheading:10604997-Antigens, CD8,
pubmed-meshheading:10604997-Cattle,
pubmed-meshheading:10604997-Clone Cells,
pubmed-meshheading:10604997-Cytokines,
pubmed-meshheading:10604997-Cytotoxicity, Immunologic,
pubmed-meshheading:10604997-Epitopes, T-Lymphocyte,
pubmed-meshheading:10604997-Female,
pubmed-meshheading:10604997-Genetic Vectors,
pubmed-meshheading:10604997-H-2 Antigens,
pubmed-meshheading:10604997-Humans,
pubmed-meshheading:10604997-Insulin,
pubmed-meshheading:10604997-Lymphocyte Activation,
pubmed-meshheading:10604997-Mice,
pubmed-meshheading:10604997-Mice, Inbred C57BL,
pubmed-meshheading:10604997-Molecular Sequence Data,
pubmed-meshheading:10604997-Peptides,
pubmed-meshheading:10604997-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:10604997-Th1 Cells,
pubmed-meshheading:10604997-Th2 Cells,
pubmed-meshheading:10604997-Transfection,
pubmed-meshheading:10604997-Tumor Cells, Cultured
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| pubmed:year |
2000
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| pubmed:articleTitle |
Bovine and human insulin activate CD8+-autoreactive CTL expressing both type 1 and type 2 cytokines in C57BL/6 mice.
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| pubmed:affiliation |
Department of Ophthalmology, Winship Cancer Center, Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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