Linked Life Data

10603370

Source:http://linkedlifedata.com/resource/pubmed/id/10603370

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-1-11
pubmed:abstractText
Pasteurella (Mannheimia) haemolytica leukotoxin (Lkt) causes cell type- and species-specific effects in ruminant leukocytes. Recent studies indicate that P. haemolytica Lkt binds to bovine CD18, the common subunit of all beta2 integrins. We designed experiments with the following objectives: to identify which member of the beta2 integrins is a receptor for Lkt; to determine whether Lkt binding to the receptor is target cell (bovine leukocytes) specific; to define the relationships between Lkt binding to the receptor, calcium elevation, and cytolysis; and to determine whether a correlation exists between Lkt receptor expression and the magnitude of target cell cytolysis. We compared Lkt-induced cytolysis in neutrophils from control calves and from calves with bovine leukocyte adhesion deficiency (BLAD), because neutrophils from BLAD-homozygous calves exhibit reduced beta2 integrin expression. The results demonstrate for the first time that Lkt binds to bovine CD11a and CD18 (lymphocyte function-associated antigen 1 [LFA-1]). The binding was abolished by anti-CD11a or anti-CD18 monoclonal antibody (MAb). Lkt-induced calcium elevation in bovine alveolar macrophages (BAMs) was inhibited by anti-CD11a or anti-CD18 MAb (65 to 94% and 37 to 98%, respectively, at 5 and 50 Lkt units per ml; P < 0.05). Lkt-induced cytolysis in neutrophils and BAMs was also inhibited by anti-CD11a or anti-CD18 MAb in a concentration-dependent manner. Lkt bound to porcine LFA-1 but did not induce calcium elevation or cytolysis. In neutrophils from BLAD calves, Lkt-induced cytolysis was decreased by 44% compared to that of neutrophils from control calves (P < 0.05). These results indicate that LFA-1 is a Lkt receptor, Lkt binding to LFA-1 is not target cell specific, Lkt binding to bovine LFA-1 correlates with calcium elevation and cytolysis, and bovine LFA-1 expression correlates with the magnitude of Lkt-induced target cell cytolysis.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-10222211, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-10414364, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-1548621, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-1558765, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-1605311, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-1753879, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-1949506, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-1971298, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-1978618, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-2191489, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-2464599, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-2750075, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-3594570, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-4073635, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-688077, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-7018660, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-7476090, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-7911733, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-8310662, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-8388866, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-8896223, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-9009313, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-9220577, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-9284143, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-9374538, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-9441798, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-9466945, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-9582961, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-9596757, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-9676575, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-9712747, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-9851045, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-9895270, http://linkedlifedata.com/resource/pubmed/commentcorrection/10603370-9916073
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
68
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
72-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Lymphocyte function-associated antigen 1 is a receptor for Pasteurella haemolytica leukotoxin in bovine leukocytes.
pubmed:affiliation
Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota 55108, USA.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't