Linked Life Data

10603034

Source:http://linkedlifedata.com/resource/pubmed/id/10603034

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2000-1-19
pubmed:abstractText
To investigate the effect of murine cytomegalovirus (MCMV) infection on the developing mouse brain in vitro, we developed an infection system using cerebral slice cultures. Using a micromanipulator, the cerebral slices from mouse embryos on day 18.5 of gestation were injected in the subventricular zone with recombinant MCMV in which the lacZ gene was inserted into a late gene, and were cultured for 7 days. Viral infection, detected by beta-galactosidase reaction, was developed at the injection sites of the slices. The virus-infected spots in the slices were enhanced by adding tumor necrosis factor-alpha to the medium and inhibited by adding phosphonoacetic acid or ganciclovir. Sections from paraffin-embedded slices were subjected to immunohistochemical analyses. Neuronal cells, labeled with 5-bromo-2-deoxyuridine 24 h before cutting the slices, migrated to the cerebral cortex in the slices. Virus-infected neuronal cells expressing only the early viral antigen migrated to the cortex, whereas glial cells expressing the immediate early and late antigens tended to remain at the injected sites. The neuronal migration of infected cells was not observed in the cerebral slices from 7-day-old mice and viral infection was not detected after injection in the cerebral slices from 14- and 21-day-old mice. These results from these cerebral slices may reflect the infectious dynamics in vivo, and this system may provide a useful model for analysis of disorders of brain development caused by CMV.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0001-6322
pubmed:author
pubmed:issnType
Print
pubmed:volume
98
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
590-6
pubmed:dateRevised
2007-11-9
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Migration of virus-infected neuronal cells in cerebral slice cultures of developing mouse brains after in vitro infection with murine cytomegalovirus.
pubmed:affiliation
Second Department of Pathology, Hamamatsu University School of Medicine, 3600 Handa-cho, Hamamatsu 431-3192, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't