| pubmed-article:10602019 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10602019 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:10602019 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
| pubmed-article:10602019 | lifeskim:mentions | umls-concept:C1155065 | lld:lifeskim |
| pubmed-article:10602019 | lifeskim:mentions | umls-concept:C1154382 | lld:lifeskim |
| pubmed-article:10602019 | lifeskim:mentions | umls-concept:C1514559 | lld:lifeskim |
| pubmed-article:10602019 | lifeskim:mentions | umls-concept:C0079717 | lld:lifeskim |
| pubmed-article:10602019 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:10602019 | lifeskim:mentions | umls-concept:C0812444 | lld:lifeskim |
| pubmed-article:10602019 | lifeskim:mentions | umls-concept:C0004083 | lld:lifeskim |
| pubmed-article:10602019 | lifeskim:mentions | umls-concept:C2349975 | lld:lifeskim |
| pubmed-article:10602019 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:10602019 | pubmed:dateCreated | 2000-1-4 | lld:pubmed |
| pubmed-article:10602019 | pubmed:abstractText | We previously demonstrated that CD82, expressed on both T cells and antigen-presenting cells (APC), plays an important role as a co-stimulatory molecule especially in the early phase of T cell activation. We also showed that the CD82 expression level is up-regulated on activated T cells and memory T cells. This up-regulation enhances both T cell-T cell and T cell-APC interactions. In this study, we further investigated the mechanism of CD82-mediated cell-cell adhesion. The enhanced adhesion between CD82-overexpressing Jurkat cells was completely blocked by anti-ICAM-1 / LFA-1 monoclonal antibodies. Increased interaction of LFA-1 with ICAM-1 was further confirmed by enhanced adhesion of CD82-overexpressing Jurkat cells to immobilized ICAM-1-Ig. CD82 co-immunoprecipitated with LFA-1 from Jurkat cells and CD82 and LFA-1 colocalized at an adhesion foci. These results suggest that the T cell stimulation via anti-CD3 cross-linking or phorbol myristate acetate treatment up-regulates CD82 expression, leading to the colocalization of CD82 and LFA-1, and results in enhanced interaction between LFA-1 and ICAM-1. In addition, a blocking experiment using monoclonal antibodies suggested that CD82 and LFA-1 molecules on APC are also important for the optimal activation of T cells. This is the first report that describes the enhancement of cell-cell interaction through LFA-1 and ICAM-1 by the overexpression of another cell surface molecule, CD82. | lld:pubmed |
| pubmed-article:10602019 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10602019 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10602019 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10602019 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:10602019 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10602019 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10602019 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:10602019 | pubmed:issn | 0014-2980 | lld:pubmed |
| pubmed-article:10602019 | pubmed:author | pubmed-author:ShimadaSS | lld:pubmed |
| pubmed-article:10602019 | pubmed:author | pubmed-author:YamashitaHH | lld:pubmed |
| pubmed-article:10602019 | pubmed:author | pubmed-author:HamadaHH | lld:pubmed |
| pubmed-article:10602019 | pubmed:author | pubmed-author:ShibagakiNN | lld:pubmed |
| pubmed-article:10602019 | pubmed:author | pubmed-author:HanadaK iK | lld:pubmed |
| pubmed-article:10602019 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10602019 | pubmed:volume | 29 | lld:pubmed |
| pubmed-article:10602019 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10602019 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10602019 | pubmed:pagination | 4081-91 | lld:pubmed |
| pubmed-article:10602019 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:10602019 | pubmed:meshHeading | pubmed-meshheading:10602019... | lld:pubmed |
| pubmed-article:10602019 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10602019 | pubmed:articleTitle | Overexpression of CD82 on human T cells enhances LFA-1 / ICAM-1-mediated cell-cell adhesion: functional association between CD82 and LFA-1 in T cell activation. | lld:pubmed |
| pubmed-article:10602019 | pubmed:affiliation | Department of Molecular Biotherapy Research Cancer Chemotherapy Center, Cancer, Institute, Tokyo, Japan. | lld:pubmed |
| pubmed-article:10602019 | pubmed:publicationType | Journal Article | lld:pubmed |
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