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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0037083,
umls-concept:C0039400,
umls-concept:C0076836,
umls-concept:C0086860,
umls-concept:C0597298,
umls-concept:C0597357,
umls-concept:C1145667,
umls-concept:C1199517,
umls-concept:C1335073,
umls-concept:C1419603,
umls-concept:C1710082,
umls-concept:C2717992
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pubmed:issue |
12
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pubmed:dateCreated |
2000-1-4
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pubmed:databankReference |
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pubmed:abstractText |
TEA (T early alpha) is a genetic element located upstream of the TCR-Jalpha cluster. Thymocytes from mice carrying a targeted deletion of TEA do not rearrange their TCRalpha locus on a window spanning the first nine Jalpha segments. This led us to the hypothesis of TEA having a "rearrangement focusing" activity on the 5' side of the TCR-Jalpha region. We analyzed DNAseI and "phylogenetic" footprints within the TEA promoter in an attempt to identify trans-acting factors that could account for its regulatory function on DNA accessibility. One of these footprints corresponded to a putative DNA-binding site for an orphan nuclear receptor of the ROR / RZR family. The RORgammaT cDNA clone was isolated from a thymus library using a probe corresponding to the DNA-binding domain of RORgamma / TOR. RORgammaT is a thymus-specific isoform of RORgamma, expressed almost exclusively in immature double-positive thymocytes. RORgammaT binds, to the TEA promoter in vitro. Lastly, the expression of RORgammaT is stimulated in two situations that mimic activation through the pre-TCR and in which the thymocytes have their TCR-alpha locus in an "open", yet unrearranged DNA configuration. We propose that the expression of RORgammaT may be part of the pre-TCR activation cascade leading to the maturation of alpha / beta T cells and may participate in the regulation of DNA accessibility in the TCR-Jalpha locus.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acid Transport Systems, Basic,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Receptor Subfamily 1...,
http://linkedlifedata.com/resource/pubmed/chemical/RORC protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Retinoic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Thyroid Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Rorc protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Slc7a2 protein, mouse
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0014-2980
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4072-80
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10602018-Amino Acid Transport Systems, Basic,
pubmed-meshheading:10602018-Animals,
pubmed-meshheading:10602018-Carrier Proteins,
pubmed-meshheading:10602018-Genes, Immunoglobulin,
pubmed-meshheading:10602018-Humans,
pubmed-meshheading:10602018-Membrane Proteins,
pubmed-meshheading:10602018-Mice,
pubmed-meshheading:10602018-Nuclear Receptor Subfamily 1, Group F, Member 3,
pubmed-meshheading:10602018-Promoter Regions, Genetic,
pubmed-meshheading:10602018-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:10602018-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:10602018-Receptors, Retinoic Acid,
pubmed-meshheading:10602018-Receptors, Thyroid Hormone,
pubmed-meshheading:10602018-Signal Transduction,
pubmed-meshheading:10602018-T-Lymphocytes,
pubmed-meshheading:10602018-Thymus Gland,
pubmed-meshheading:10602018-Up-Regulation
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pubmed:year |
1999
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pubmed:articleTitle |
RORgammaT, a thymus-specific isoform of the orphan nuclear receptor RORgamma / TOR, is up-regulated by signaling through the pre-T cell receptor and binds to the TEA promoter.
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pubmed:affiliation |
Développement Normal et Pathologique du Système Immunitaire INSERM U429, Hôpital Necker Enfants Malades, Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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