|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1-2
|
|
pubmed:dateCreated |
2000-2-3
|
|
pubmed:abstractText |
To obtain proximity information between tropomyosin (Tm) and caldesmon (CaD) on the muscle thin filament, we cloned gizzard alphaTm and created two single Cys mutants S56C/C190S (56Tm) and D100C/C190S (100Tm). They were labeled with benzophenone maleimide (BPM) and UV-irradiated on thin filaments. One chain of BPM-56Tm and two chains of BPM-100Tm crosslinked to CaD. Only BPM-100Tm crosslinked to actin in the absence and presence of CaD and binding of low ratios of myosin subfragment 1 (S1) prevented the crosslinking. Tm-S1 crosslinks were produced when actin.Tm was saturated with S1. Thus, CaD on the actin.Tm filament is located <10 A away from Tm amino acids 56 and 100; in the closed state of the actin.Tm filament, Tm residue 100 is located close to the actin surface and is moved further away in the S1-induced open state; in the open state, S1 binds close to Tm.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0014-5793
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
10
|
|
pubmed:volume |
463
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
146-50
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:10601656-Actins,
pubmed-meshheading:10601656-Animals,
pubmed-meshheading:10601656-Calmodulin-Binding Proteins,
pubmed-meshheading:10601656-Chickens,
pubmed-meshheading:10601656-Densitometry,
pubmed-meshheading:10601656-Escherichia coli,
pubmed-meshheading:10601656-Kinetics,
pubmed-meshheading:10601656-Muscle, Smooth,
pubmed-meshheading:10601656-Muscle Fibers, Skeletal,
pubmed-meshheading:10601656-Mutagenesis,
pubmed-meshheading:10601656-Myosin Subfragments,
pubmed-meshheading:10601656-Protein Binding,
pubmed-meshheading:10601656-Recombinant Proteins,
pubmed-meshheading:10601656-Tropomyosin,
pubmed-meshheading:10601656-Ultraviolet Rays
|
|
pubmed:year |
1999
|
|
pubmed:articleTitle |
Smooth muscle alpha-tropomyosin crosslinks to caldesmon, to actin and to myosin subfragment 1 on the muscle thin filament.
|
|
pubmed:affiliation |
Muscle Research Group, Boston Biomedical Research Institute, Boston, MA 02115, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
