Linked Life Data

10601578

Source:http://linkedlifedata.com/resource/pubmed/id/10601578

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-5-10
pubmed:abstractText
Endothelial cells have ectonucleotidases that catabolize extracellular nucleotides and are sensitive to the presence of cations. Our aim was to determine whether the metabolism of extracellular nucleotides is influenced by exposure of endothelial cells to high potassium relevant to human pathophysiology. Human umbilical vein endothelial cells were incubated with 25 mM potassium and without potassium. The metabolism of radioactive substrates was measured and activities were 7.0+/-1.3 and 12.2+/-1.4 microM P/h/mg of protein with and without potassium. Also incubation with membrane pellet from endothelial cells was used, this assay showed 15.5+/-1.9 and 20.9+/-0.7 microM P/ h/mg of protein with and without potassium, respectively. HPLC analysis of supernatant showed that AMP production was lower in the presence of 25 mM potassium. Analysis of different potassium levels showed that a progressive reduction occurred above 10 mM potassium. We conclude those potassium levels above 10 mM, which can be found in ischemia, inhibit the catabolism of extracellular adenine nucleotides by the ectonucleotidases of endothelial cells and may thus modify the pathophysiology of ischemia-reperfusion injury.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1107-3756
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
71-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
The role of potassium in the regulation of adenosine production by ATP-diphosphohydrolase on the endothelial cell membrane.
pubmed:affiliation
Departamento de Clinica Medica da Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo, Brazil.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't