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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2000-1-11
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pubmed:abstractText |
Protein kinase A (PKA) and the nuclear A-kinase-anchoring protein AKAP95 have previously been shown to localize in separate compartments in interphase but associate at mitosis. We demonstrate here a role for the mitotic AKAP95-PKA complex. In HeLa cells, AKAP95 is associated with the nuclear matrix in interphase and redistributes mostly into a chromatin fraction at mitosis. In a cytosolic extract derived from mitotic cells, AKAP95 recruits the RIIalpha regulatory subunit of PKA onto chromatin. Intranuclear immunoblocking of AKAP95 inhibits chromosome condensation at mitosis and in mitotic extract in a PKA-independent manner. Immunodepletion of AKAP95 from the extract or immunoblocking of AKAP95 at metaphase induces premature chromatin decondensation. Condensation is restored in vitro by a recombinant AKAP95 fragment comprising the 306-carboxy-terminal amino acids of the protein. Maintenance of condensed chromatin requires PKA binding to chromatin-associated AKAP95 and cAMP signaling through PKA. Chromatin-associated AKAP95 interacts with Eg7, the human homologue of Xenopus pEg7, a component of the 13S condensin complex. Moreover, immunoblocking nuclear AKAP95 inhibits the recruitment of Eg7 to chromatin in vitro. We propose that AKAP95 is a multivalent molecule that in addition to anchoring a cAMP/PKA-signaling complex onto chromosomes, plays a role in regulating chromosome structure at mitosis.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-10036247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-10199406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-10354567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-10460410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-1653962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-179994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-1846085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-1860836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-2172257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-2847055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-7929577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-7937913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-7954811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-8125992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-8276886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-8391536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-8408207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-8596931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-8991085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-9013715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-9128241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-9160743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-9473338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-9547309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-9570761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-9811564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-9852131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-9852142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601332-9854313
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AKAP8L protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Extracts,
http://linkedlifedata.com/resource/pubmed/chemical/Chromatin,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP-Dependent Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Eg7 protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/Egg Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Nocodazole,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Xenopus Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-9525
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
147
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1167-80
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10601332-Antibodies,
pubmed-meshheading:10601332-Cell Cycle,
pubmed-meshheading:10601332-Cell Cycle Proteins,
pubmed-meshheading:10601332-Cell Extracts,
pubmed-meshheading:10601332-Chromatin,
pubmed-meshheading:10601332-Chromosomes, Human,
pubmed-meshheading:10601332-Cyclic AMP,
pubmed-meshheading:10601332-Cyclic AMP-Dependent Protein Kinases,
pubmed-meshheading:10601332-DNA-Binding Proteins,
pubmed-meshheading:10601332-Egg Proteins,
pubmed-meshheading:10601332-Fluorescent Antibody Technique,
pubmed-meshheading:10601332-HeLa Cells,
pubmed-meshheading:10601332-Humans,
pubmed-meshheading:10601332-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:10601332-Mitosis,
pubmed-meshheading:10601332-Nocodazole,
pubmed-meshheading:10601332-Nuclear Matrix,
pubmed-meshheading:10601332-Nuclear Proteins,
pubmed-meshheading:10601332-Peptide Fragments,
pubmed-meshheading:10601332-Precipitin Tests,
pubmed-meshheading:10601332-Protein Binding,
pubmed-meshheading:10601332-Signal Transduction,
pubmed-meshheading:10601332-Xenopus Proteins
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pubmed:year |
1999
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pubmed:articleTitle |
The A-kinase-anchoring protein AKAP95 is a multivalent protein with a key role in chromatin condensation at mitosis.
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pubmed:affiliation |
Institute of Medical Biochemistry, Faculty of Medicine, University of Oslo, Blindern, 0317 Oslo, Norway. philippe.collas@basalmed.uio.no
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pubmed:publicationType |
Journal Article
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