Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
24
pubmed:dateCreated
2000-1-13
pubmed:databankReference
pubmed:abstractText
The gltA gene, encoding Sinorhizobium meliloti 104A14 citrate synthase, was isolated by complementing an Escherichia coli gltA mutant. The S. meliloti gltA gene was mutated by inserting a kanamycin resistance gene and then using homologous recombination to replace the wild-type gltA with the gltA::kan allele. The resulting strain, CSDX1, was a glutamate auxotroph, and enzyme assays confirmed the absence of a requirement for glutamate. CSDX1 did not grow on succinate, malate, aspartate, pyruvate, or glucose. CSDX1 produced an unusual blue fluorescence on medium containing Calcofluor, which is different from the green fluorescence found with 104A14. High concentrations of arabinose (0.4%) or succinate (0. 2%) restored the green fluorescence to CSDX1. High-performance liquid chromatography analyses showed that CSDX1 produced partially succinylated succinoglycan. CSDX1 was able to form nodules on alfalfa, but these nodules were not able to fix nitrogen. The symbiotic defect of a citrate synthase mutant could thus be due to disruption of the infection process or to the lack of energy generated by the tricarboxylic acid cycle.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601220-11902715, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601220-1320616, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601220-1400238, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601220-16593058, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601220-2287269, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601220-2824062, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601220-2842307, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601220-3104207, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601220-3403505, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601220-6125502, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601220-6137474, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601220-6330052, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601220-6853448, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601220-8170393, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601220-8343955, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601220-8385665, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601220-8486283, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601220-8526514, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601220-9245810
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
181
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7608-13
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Citrate synthase mutants of Sinorhizobium meliloti are ineffective and have altered cell surface polysaccharides.
pubmed:affiliation
Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.