Linked Life Data

10600490

Source:http://linkedlifedata.com/resource/pubmed/id/10600490

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2000-1-11
pubmed:abstractText
The K-sam gene was first identified as an amplified gene in the poorly differentiated types, especially in the scirrhous type, of gastric cancers. We have recently found and reported that the carboxyl-terminal exons of K-sam are frequently deleted in the scirrhous type of gastric cancer. The deletion generates preferential expression of at least six novel K-sam-II mRNAs: K-sam-IIH1, -IIH2 and -IIH3/O4, and K-sam-IIO1, -IIO2, and -IIO3, which encode novel proteins lacking the transformation-inhibitory sequence or activated K-sam proteins. In this study, we investigated expression of the previously described K-sam-IIC1 and -IIC3 mRNAs and the novel six K-sam-II mRNAs in 14 gastric cancer cell lines, 7 breast cancer cell lines, and 20 human normal tissues. All the six novel K-sam-II mRNAs were expressed preferentially in the cell lines derived from the scirrhous type of gastric cancers but not in the 7 breast cancer cell lines and the 20 human normal tissues. We further determined the positional relationship of four exons of H1, O1, O2, and O3 out of the six exons of H1, H2, H3/O4, O1, O2, and O3, and found that these four novel K-sam exons were located more than 200 kb downstream of the previously described carboxyl-terminal exon of the K-sam gene. Expression of K-sam-IIH1, -IIO1, and -IIO2 mRNAs encoding activated K-sam products in the scirrhous type of gastric cancer cell lines HSC39, OCUM2M, HSC59, and HSC60 was not due to the deletion of the C1 exon of K-sam.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-291X
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Academic Press.
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
265
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
739-45
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10600490-Amino Acid Sequence, pubmed-meshheading:10600490-Base Sequence, pubmed-meshheading:10600490-Breast Neoplasms, pubmed-meshheading:10600490-Chromosome Mapping, pubmed-meshheading:10600490-DNA Primers, pubmed-meshheading:10600490-Exons, pubmed-meshheading:10600490-Female, pubmed-meshheading:10600490-Gene Expression, pubmed-meshheading:10600490-Humans, pubmed-meshheading:10600490-Molecular Sequence Data, pubmed-meshheading:10600490-RNA, Messenger, pubmed-meshheading:10600490-RNA, Neoplasm, pubmed-meshheading:10600490-Receptor, Fibroblast Growth Factor, Type 2, pubmed-meshheading:10600490-Receptor Protein-Tyrosine Kinases, pubmed-meshheading:10600490-Receptors, Fibroblast Growth Factor, pubmed-meshheading:10600490-Receptors, Growth Factor, pubmed-meshheading:10600490-Stomach Neoplasms, pubmed-meshheading:10600490-Tumor Cells, Cultured
pubmed:year
1999
pubmed:articleTitle
Novel exons located more than 200 kb downstream of the previously described 3' exon of the K-sam gene for generating activated forms of KGF receptor.
pubmed:affiliation
Genetics Division, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo, 104-0045, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't