Linked Life Data

10600031

Source:http://linkedlifedata.com/resource/pubmed/id/10600031

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2000-1-24
pubmed:abstractText
Our previous in vitro studies indicate a significant role for cell adhesion molecules in the biology of HIV-1 and HTLV-1. Confirmation of the involvement of these molecules in the pathogenesis of retrovirus infection in vivo will require a suitable animal model. The SIV/pigtailed macaque (Macaca nemestrina) model of acquired immunodeficiency syndrome (AIDS) is an ideal system in which to study adhesion molecules and viral pathogenesis. The monoclonal antibodies (MAbs) against human adhesion molecules previously produced in our laboratory either do not react with or fail to block function of pigtailed macaque adhesion molecules. We have used papiovirus-transformed pigtailed macaque B cells as immunogen to generate murine MAbs against macaque adhesion molecules including ICAM-1, VCAM-1, and LFA-1. The specificity of the MAbs was confirmed by immunoprecipitation from lysates of vectorially iodinated cells, flow cytometry analysis of transfected cell lines and primary cells, binding assays on recombinant soluble human VCAM-1 and ICAM-1, and by inhibition of adhesion functions. MAbs against ICAM-1 and VCAM-1 showed positive staining of fixed tissue in immunohistochemistry studies. The same antibodies also blocked the function of these two adhesion molecules. The new MAbs can be used to study the tissue expression of adhesion molecules in SIV-infected animals as well as to test the involvement of these molecules in virus infection. Thus they should prove invaluable as probes of the role of cell adhesion molecules in AIDS pathogenesis in an animal model.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0272-457X
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
437-47
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10600031-Acquired Immunodeficiency Syndrome, pubmed-meshheading:10600031-Animals, pubmed-meshheading:10600031-Antibodies, Monoclonal, pubmed-meshheading:10600031-Cell Adhesion Molecules, pubmed-meshheading:10600031-Cell Line, pubmed-meshheading:10600031-Disease Models, Animal, pubmed-meshheading:10600031-Humans, pubmed-meshheading:10600031-Hybridomas, pubmed-meshheading:10600031-Immunohistochemistry, pubmed-meshheading:10600031-Intercellular Adhesion Molecule-1, pubmed-meshheading:10600031-Lymphocyte Function-Associated Antigen-1, pubmed-meshheading:10600031-Macaca nemestrina, pubmed-meshheading:10600031-Mice, pubmed-meshheading:10600031-Recombinant Proteins, pubmed-meshheading:10600031-Species Specificity, pubmed-meshheading:10600031-Transfection, pubmed-meshheading:10600031-Vascular Cell Adhesion Molecule-1
pubmed:year
1999
pubmed:articleTitle
Production and characterization of monoclonal antibodies against pigtailed macaque (Macaca nemestrina) cell adhesion molecules.
pubmed:affiliation
Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. jhildret@jhmi.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.