Source:http://linkedlifedata.com/resource/pubmed/id/10599917
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
|
| pubmed:dateCreated |
2000-1-24
|
| pubmed:databankReference | |
| pubmed:abstractText |
A cDNA clone that encodes a Plasmodium falciparum asparagine (N)-rich protein (PfARP) was isolated through immunoscreening of an expression library. A 9.4 kb PfARP transcript was identified by Northern blot hybridization and the gene was localized on chromosome 1. The complete coding sequence (6666 bp) revealed a protein that contains clustered as well as randomly distributed N residues (24.3%), seven copies of a repeat sequence [DNT(D/N)(K/N)(V/L/M)] and multiple copies of tripeptide repeats within a 101 amino acid region containing 89 D/E residues. The PfARP was immunogenic in inbred and outbred mice and endemic sera revealed the presence of low-titer antibodies against PfARP. Anti-PfARP sera showed cytoplasmic and surface localization of apparently cross-reactive malarial antigens in different life-cycle stages (ring, trophozoite, schizont, and gametocytes). Although the biological function(s) of PfARP are not known, the observation that it is present in multiple parasite stages and that it is a target of natural immune response warrants further study of PfARP as an immune target.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0932-0113
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
85
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
956-63
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:10599917-Adolescent,
pubmed-meshheading:10599917-Adult,
pubmed-meshheading:10599917-Amino Acid Sequence,
pubmed-meshheading:10599917-Animals,
pubmed-meshheading:10599917-Antibodies, Protozoan,
pubmed-meshheading:10599917-Antigens, Protozoan,
pubmed-meshheading:10599917-Child,
pubmed-meshheading:10599917-Child, Preschool,
pubmed-meshheading:10599917-Cloning, Molecular,
pubmed-meshheading:10599917-Cross Reactions,
pubmed-meshheading:10599917-DNA, Complementary,
pubmed-meshheading:10599917-Gene Library,
pubmed-meshheading:10599917-Humans,
pubmed-meshheading:10599917-Immunization,
pubmed-meshheading:10599917-Immunoblotting,
pubmed-meshheading:10599917-Infant,
pubmed-meshheading:10599917-Malaria, Falciparum,
pubmed-meshheading:10599917-Mice,
pubmed-meshheading:10599917-Molecular Sequence Data,
pubmed-meshheading:10599917-Plasmodium falciparum,
pubmed-meshheading:10599917-Recombinant Proteins,
pubmed-meshheading:10599917-Sequence Analysis, DNA
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Cloning and characterization of a new asparagine-rich protein in Plasmodium falciparum.
|
| pubmed:affiliation |
Molecular Microbiology and Immunology, Johns Hopkins University School of Public Health, Baltimore, MD 21205, USA.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|