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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1976-2-13
|
| pubmed:abstractText |
Dihydrofolate reductase activity of 0.2 nmole of dihydrofolate reduced/min/mg protein was detected in crude extracts of unsporulated oocysts of Eimeria tenella. The enzyme was purified by a combination of affinity and ion-exchange chromatography. Its molecular weight was estimated as 240,000 daltons. An anticoccidial drug pyrimethamine is a potent inhibitor of the activity of E. tenella dihydrofolate reductase (Ki = 3 nM), but it is less effective an inhibitor of dihydrofolate reductase from chicken liver. This difference may explain the in vivo therapeutic action of pyrimethamine against Coccidia.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0022-3921
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
22
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
564-8
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1059772-Animals,
pubmed-meshheading:1059772-Chickens,
pubmed-meshheading:1059772-Eimeria,
pubmed-meshheading:1059772-Folic Acid Antagonists,
pubmed-meshheading:1059772-Liver,
pubmed-meshheading:1059772-Methotrexate,
pubmed-meshheading:1059772-Molecular Weight,
pubmed-meshheading:1059772-Pyrimethamine,
pubmed-meshheading:1059772-Tetrahydrofolate Dehydrogenase
|
| pubmed:year |
1975
|
| pubmed:articleTitle |
Dihydrofolate reductase from Eimeria tenella: rationalization of chemotherapeutic efficacy of pyrimethamine.
|
| pubmed:publicationType |
Journal Article
|