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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2000-1-6
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pubmed:abstractText |
To determine whether vascular endothelial growth factor (VEGF)-induced tumor microvascularity is detectable by in vivo NMR imaging, an experimental study was conducted in nude mice. Human breast cancer cells (MCF-7) and MCF-7 cells stably transfected with the cDNA for the VEGF165 isoform (MV165) were grown in nude mice and models were characterized by RT-PCR, Western blotting, ELISA, immunohistochemistry and NMR imaging using a novel synthetic protected graft copolymer (PGC) as a vascular probe. MV165 tumors showed a 1.6-fold higher microvascular density by histology. Both tumors showed identical MR signal intensities on non-contrast and Gd-DTPA enhanced images. PGC enhanced MR imaging of tumoral vascular volume fraction (VVF), however, revealed significant differences between the 2 tumor types (MV165: 8.9 +/- 2.1; MCF-7: 1.7 +/- 0.5; p < 0.003), as expected from histology. VVF changes were more heterogeneous in the MV165 model both among tumors as well as within tumors as determined 3-dimensionally at submillimeter resolutions. Our results have potential applications for non-invasive assessment of angiogenesis by in vivo imaging and for clinical monitoring during angiogenic therapies.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0020-7136
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
798-802
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10597197-Animals,
pubmed-meshheading:10597197-Breast Neoplasms,
pubmed-meshheading:10597197-Endothelial Growth Factors,
pubmed-meshheading:10597197-Female,
pubmed-meshheading:10597197-Gadolinium DTPA,
pubmed-meshheading:10597197-Humans,
pubmed-meshheading:10597197-Lymphokines,
pubmed-meshheading:10597197-Magnetic Resonance Spectroscopy,
pubmed-meshheading:10597197-Mice,
pubmed-meshheading:10597197-Mice, Nude,
pubmed-meshheading:10597197-Neovascularization, Pathologic,
pubmed-meshheading:10597197-RNA, Messenger,
pubmed-meshheading:10597197-Recombinant Proteins,
pubmed-meshheading:10597197-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10597197-Transcription, Genetic,
pubmed-meshheading:10597197-Transfection,
pubmed-meshheading:10597197-Transplantation, Heterologous,
pubmed-meshheading:10597197-Tumor Cells, Cultured,
pubmed-meshheading:10597197-Vascular Endothelial Growth Factor A,
pubmed-meshheading:10597197-Vascular Endothelial Growth Factors
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pubmed:year |
1999
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pubmed:articleTitle |
In vivo assessment of vascular endothelial growth factor-induced angiogenesis.
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pubmed:affiliation |
Center for Molecular Imaging Research, Massachusetts General Hospital/Harvard Medical School, Charlestown 02129, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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