Source:http://linkedlifedata.com/resource/pubmed/id/10594353
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0001175,
umls-concept:C0009013,
umls-concept:C0024398,
umls-concept:C0026336,
umls-concept:C0026339,
umls-concept:C0031809,
umls-concept:C0039194,
umls-concept:C0039195,
umls-concept:C0175727,
umls-concept:C0206558,
umls-concept:C0449435,
umls-concept:C0871261,
umls-concept:C1332714,
umls-concept:C1521840,
umls-concept:C1524063,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C2911692
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| pubmed:issue |
1-2
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| pubmed:dateCreated |
2000-1-7
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| pubmed:abstractText |
Herpesvirus saimiri (HVS), a nonhuman primate gamma herpes virus, was used to immortalize pig-tailed macaque CD4(+) T lymphocytes. The HVS-immortalized T cell lines were used to develop CD4(+) T cell clones from two animals. Three CD4(+) T cell clones were further characterized for the expression of cell surface markers. All expressed CD2, CD4, CD58, CD69 and CD80 and therefore resembled activated T cells. These clones required exogenous IL-2 for efficient growth and were found to be highly susceptible to infection by the challenge virus, Chimeric simian/human immunodeficiency virus (SHIV(KU-1)). They could also be productively infected not only by the quasispecies of the challenge virus (SHIV(KU-1/PDJ) and SHIV(KU-1/PNA), isolated from macaque PDj and PNa, respectively) but also by a different chimeric simian/human immunodeficiency virus (SHIV(89.6P)) and simian immunodeficiency virus (SIV(MAC239)). The virus-infected CD4(+) T cell clones were also used as stimulators for generation of CTL effectors. These effectors exhibited excellent virus-specific lysis in chromium-release assays when syngenic SHIV(KU-1) infected autologous CD4(+) T cell clones were used as targets. The target cell lysis was virus specific, as uninfected control cells showed no or minimal lysis.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
0022-1759
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
19
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| pubmed:volume |
230
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
47-58
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:10594353-Acquired Immunodeficiency Syndrome,
pubmed-meshheading:10594353-Animals,
pubmed-meshheading:10594353-Antigens, CD,
pubmed-meshheading:10594353-Antigens, Viral,
pubmed-meshheading:10594353-CD4-Positive T-Lymphocytes,
pubmed-meshheading:10594353-Cell Transformation, Viral,
pubmed-meshheading:10594353-Chimera,
pubmed-meshheading:10594353-Clone Cells,
pubmed-meshheading:10594353-Cytotoxicity Tests, Immunologic,
pubmed-meshheading:10594353-Disease Models, Animal,
pubmed-meshheading:10594353-HIV,
pubmed-meshheading:10594353-HIV Antigens,
pubmed-meshheading:10594353-Herpesvirus 2, Saimiriine,
pubmed-meshheading:10594353-Humans,
pubmed-meshheading:10594353-Interleukin-2,
pubmed-meshheading:10594353-Macaca nemestrina,
pubmed-meshheading:10594353-Simian Acquired Immunodeficiency Syndrome,
pubmed-meshheading:10594353-Simian immunodeficiency virus,
pubmed-meshheading:10594353-T-Lymphocytes, Cytotoxic
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| pubmed:year |
1999
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| pubmed:articleTitle |
Use of herpesvirus saimiri-immortalized macaque CD4(+) T cell clones as stimulators and targets for assessment of CTL responses in macaque/AIDS models.
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| pubmed:affiliation |
Marion Merrell Dow Laboratory of Viral Pathogenesis and Department of Microbiology, Molecular Genetics and Immunology, University Kansas Medical Center, Kansas City, KS 66160, USA. akumar@kumc.edu
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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