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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2000-2-14
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pubmed:abstractText |
The aim of this study was to characterize the effects of auranofin (2,3,4,6-tetra-O-acetyl-l-thio-beta-D-gluco-pyranosato-S) on cyclooxygenase expression and prostaglandin E(2) synthesis on cultured human synovial fibroblast-like cells (synoviocytes). Synoviocytes were treated with auranofin in the presence or absence of interleukin-1beta. Cultured supernatants were harvested for prostaglandin E(2) synthesis. Cyclooxygenase-1 and -2 expression was analyzed with Western and Northern blotting. Translocation of nuclear factor-kappa B p65 was determined by immunostaining. Cytotoxicity was measured with 51Cr release assay. Auranofin attenuated interleukin-1beta-induced prostaglandin E(2) production of the cells in a dose-dependent fashion. Auranofin selectively suppressed interleukin-1beta-induced cyclooxygenase-2 mRNA and protein expression of the cells without alteration of cyclooxygenase-1 expression. Also, auranofin interfered with interleukin-1beta-induced translocation of nuclear factor-kappa B. These inhibitory effects did not originate in the cytotoxicity of the agent. Our data indicate that auranofin inhibits interleukin-1beta-induced prostaglandin E(2) synthesis and cyclooxygenase-2 expression via suppression of nuclear factor-kappa B activation on synoviocytes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Auranofin,
http://linkedlifedata.com/resource/pubmed/chemical/Chromium Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Dexamethasone,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/PTGS1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/PTGS2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin-Endoperoxide Synthases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0014-2999
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
385
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
71-9
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:10594346-Auranofin,
pubmed-meshheading:10594346-Blotting, Northern,
pubmed-meshheading:10594346-Blotting, Western,
pubmed-meshheading:10594346-Cell Survival,
pubmed-meshheading:10594346-Cells, Cultured,
pubmed-meshheading:10594346-Chromium Radioisotopes,
pubmed-meshheading:10594346-Cyclooxygenase 1,
pubmed-meshheading:10594346-Cyclooxygenase 2,
pubmed-meshheading:10594346-Dexamethasone,
pubmed-meshheading:10594346-Dinoprostone,
pubmed-meshheading:10594346-Dose-Response Relationship, Drug,
pubmed-meshheading:10594346-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:10594346-Humans,
pubmed-meshheading:10594346-Interleukin-1,
pubmed-meshheading:10594346-Isoenzymes,
pubmed-meshheading:10594346-Membrane Proteins,
pubmed-meshheading:10594346-NF-kappa B,
pubmed-meshheading:10594346-Prostaglandin-Endoperoxide Synthases,
pubmed-meshheading:10594346-RNA, Messenger,
pubmed-meshheading:10594346-Synovial Membrane,
pubmed-meshheading:10594346-Transcription, Genetic
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pubmed:year |
1999
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pubmed:articleTitle |
Auranofin inhibits interleukin-1beta-induced transcript of cyclooxygenase-2 on cultured human synoviocytes.
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pubmed:affiliation |
First Department of Internal Medicine, Kyoto Prefectural University of Medicine, 465 Kajiicho, Kamigyo-ku, Kyoto, Japan.
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pubmed:publicationType |
Journal Article
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