Linked Life Data

10591972

Source:http://linkedlifedata.com/resource/pubmed/id/10591972

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2000-10-24
pubmed:databankReference
pubmed:abstractText
From a set of Phytophthora infestans cDNA clones that were randomly selected from a potato- P. infestans interaction cDNA library, a relatively high proportion (5 out of 22) appeared to be derived from the same gene. The gene was designated ric1. P. infestans contains two copies of ric1 which share 98% homology at the nucleotide-sequence level and 100% at the amino-acid level. The nucleotide sequence predicts an open reading frame of 171 bp encoding a 57 amino-acid hydrophobic-peptide with two potential membrane-spanning domains. The predicted peptide shows high homology to a peptide encoded by plant genes whose expression is specifically induced during stress conditions. Southern-blot analysis of genomic DNA of several Phytophthora species indicated that most species contain ric1 homologues. During the life cycle of P. infestans, ric1 was expressed in all developmental stages but the level of expression varied. Sporangia and germinating cysts appeared to contain only very little ric1 mRNA whereas in the mycelium and during in planta growth higher levels were detected. Subjecting the mycelium to osmotic stress or to a high pH resulted in increased ric1 expression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0172-8083
pubmed:author
pubmed:issnType
Print
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
310-5
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Ric1, a Phytophthora infestans gene with homology to stress-induced genes.
pubmed:affiliation
Laboratory of Phytopathology, Wageningen Agricultural University and Graduate School of Experimental Plant Sciences, Binnenhaven 9, 6709 PD Wageningen, The Netherlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't