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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4-5
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pubmed:dateCreated |
2000-1-3
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pubmed:abstractText |
Appropriate regulation of tyrosine phosphorylation is essential for axon growth and guidance; evidence from invertebrates indicates that receptor-type tyrosine phosphatases (RPTPs) are required for correct axon growth during CNS development. One vertebrate RPTP, PTP-delta, is highly expressed in brain and has a cell adhesion molecule-like extracellular domain (ECD) comprising three immunoglobulin repeats and eight fibronectin type III repeats. Using fluorescent beads (Covaspheres) coated with the PTP-delta ECD, as well as insect cells expressing PTP-delta on their surfaces, we show that PTP-delta is a homophilic cell adhesion molecule. A variety of chick neurons adhere strongly to an Fc fusion protein containing the PTP-delta ECD. Additionally, substrate-bound PTP-delta ECD fusion protein strongly promotes neurite outgrowth from forebrain neurons; this effect is separable from its effect on adhesion. Our results indicate that PTP-delta is a neurite-promoting cell adhesion molecule for CNS neurons.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1044-7431
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
370-84
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:10588391-Animals,
pubmed-meshheading:10588391-Baculoviridae,
pubmed-meshheading:10588391-CHO Cells,
pubmed-meshheading:10588391-Catalytic Domain,
pubmed-meshheading:10588391-Cell Adhesion,
pubmed-meshheading:10588391-Cell Adhesion Molecules, Neuronal,
pubmed-meshheading:10588391-Chick Embryo,
pubmed-meshheading:10588391-Cloning, Molecular,
pubmed-meshheading:10588391-Cricetinae,
pubmed-meshheading:10588391-Gene Expression Regulation, Developmental,
pubmed-meshheading:10588391-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:10588391-Humans,
pubmed-meshheading:10588391-Molecular Sequence Data,
pubmed-meshheading:10588391-Neurites,
pubmed-meshheading:10588391-Neurons,
pubmed-meshheading:10588391-Prosencephalon,
pubmed-meshheading:10588391-Protein Binding,
pubmed-meshheading:10588391-Protein Structure, Tertiary,
pubmed-meshheading:10588391-Protein Tyrosine Phosphatases,
pubmed-meshheading:10588391-RNA, Messenger,
pubmed-meshheading:10588391-Receptor-Like Protein Tyrosine Phosphatases, Class 2,
pubmed-meshheading:10588391-Recombinant Fusion Proteins,
pubmed-meshheading:10588391-Sequence Homology, Amino Acid,
pubmed-meshheading:10588391-Transfection
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pubmed:articleTitle |
Receptor tyrosine phosphatase-delta is a homophilic, neurite-promoting cell adhesion molecular for CNS neurons.
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pubmed:affiliation |
Department of Molecular and Cellular Pharmacology, University of Miami School of Medicine, Florida 33101, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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