Linked Life Data

10587104

Source:http://linkedlifedata.com/resource/pubmed/id/10587104

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1999-12-30
pubmed:abstractText
The single photon emission computed tomography (SPECT) D2/D3 receptor radiotracer [123I]IBZM, is prepared by electrophilic radioiodination of the precursor BZM with high-purity sodium [123I]iodide in the presence of diluted peracetic acid. However, in our hands, the most commonly used procedure for this radiosynthesis produced variable and inconsistent labeling yields, to such extent that it became inappropriate for routine clinical studies. Our goal was to modify the labeling procedure, to obtain consistently better labeling and radiochemical yields. The best conditions found for the radioiodination were as follows: 50 microg precursor in 50 microL EtOH mixed with buffer pH 2; Na[123I]I in 0.1 M NaOH (< 180 microL), 50 microL peracetic acid diluted solution, heating at 65 degrees C for 14 min. Purification was achieved by solid phase extraction (SPE) and reverse-phase high performance liquid chromatography (HPLC). Under these conditions, labeling yield average was 76 +/- 4% (n = 31); radiochemical yield was 69 +/- 4% and radiochemical purity was 98 +/- 1%. With larger volumes of the Na[123I]I solution the yields were consistent but lower. For example, for volumes between 417 and 523 microL the labeling yield was 61 +/- 5% (n = 21), radiochemical yield was 56 +/- 5% and radiochemical purity was 98 +/- 1%.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0969-8051
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
661-5
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Synthesis of [123I]IBZM: a reliable procedure for routine clinical studies.
pubmed:affiliation
Department of Psychiatry, Columbia University College of Physicians and Surgeons, and New York State Psychiatric Institute, New York, USA. yolanda@neuron.cpmc.columbia.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.