Linked Life Data

10586035

Source:http://linkedlifedata.com/resource/pubmed/id/10586035

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2000-1-6
pubmed:abstractText
Previous studies demonstrate that aluminium hydroxide adjuvant (alum) produces increased Th1 responses in IL-4-deficient mice compared with wild-type animals, although the continued production of IL-5 by spleen cells from these mice also indicates that Th2 responses are induced. In the present study, we demonstrate that alum can induce Th2-associated IL-4 and IL-5 production in the absence of IL-4 signaling in mice deficient in either IL-4Ralpha or Stat6. The Th2 responses observed could not be due to IL-13 as IL-13 responses are also impaired in IL-4Ralpha- and Stat6-deficient mice. We also detected higher levels of IL-4 in IL-4Ralpha gene-deficient, though not Stat6-deficient, mice compared with their wild-type counterparts. The increased levels of IL-4 could be explained by the IL-4R being unavailable to neutralize this cytokine in IL-4Ralpha-deficient mice. While levels of IL-5 production in IL-4Ralpha- or Stat6-deficient mice were similar to IL-4-deficient and wild-type mice, other type 2-associated responses, which are largely or wholly IL-4 dependent, such as the production of IgG1 or IgE Abs, were either reduced or absent. We conclude that alum adjuvants can induce IL-4 production and Th2 responses independently of IL-4 or IL-13, negating the requirement for an early source of IL-4 in the Th2 response induced by this adjuvant.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
163
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6448-54
pubmed:dateRevised
2010-8-25
pubmed:meshHeading
pubmed-meshheading:10586035-Adjuvants, Immunologic, pubmed-meshheading:10586035-Aluminum Hydroxide, pubmed-meshheading:10586035-Animals, pubmed-meshheading:10586035-Cells, Cultured, pubmed-meshheading:10586035-Cytokines, pubmed-meshheading:10586035-Epitopes, T-Lymphocyte, pubmed-meshheading:10586035-Female, pubmed-meshheading:10586035-Immunoglobulin G, pubmed-meshheading:10586035-Interleukin-13, pubmed-meshheading:10586035-Interleukin-4, pubmed-meshheading:10586035-Lymphocyte Activation, pubmed-meshheading:10586035-Mice, pubmed-meshheading:10586035-Mice, Inbred BALB C, pubmed-meshheading:10586035-Mice, Inbred C57BL, pubmed-meshheading:10586035-Mice, Knockout, pubmed-meshheading:10586035-Ovalbumin, pubmed-meshheading:10586035-Signal Transduction, pubmed-meshheading:10586035-Solubility, pubmed-meshheading:10586035-Th2 Cells
pubmed:year
1999
pubmed:articleTitle
Aluminium hydroxide adjuvant initiates strong antigen-specific Th2 responses in the absence of IL-4- or IL-13-mediated signaling.
pubmed:affiliation
Department of Immunology, University of Glasgow, Western Infirmary, Scotland, United Kingdom. j.m.brewer@clinmed.gla.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't