rdf:type |
|
lifeskim:mentions |
umls-concept:C0009063,
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0020284,
umls-concept:C0185117,
umls-concept:C0679058,
umls-concept:C1521991,
umls-concept:C1547699,
umls-concept:C1880022,
umls-concept:C2700640,
umls-concept:C2911684
|
pubmed:issue |
2
|
pubmed:dateCreated |
2000-2-10
|
pubmed:databankReference |
|
pubmed:abstractText |
A putative hydrogenase (hydA) gene of Clostridium perfringens encodes a protein with strong identity to Clostridium pasteurianum hydrogenase I. Disruption of the hydA gene abolished H(2) productivity, confirming its function. A putative butyrate kinase gene (buk) is adjacent to the hydA gene. When cultures were grown in medium with glucose, 1.8-kb hydA and 2.1-kb buk transcripts and a 3. 9-kb transcript hybridized with both hydA and buk-probe were detectable in all the exponential growth phases. In medium without glucose, these transcripts were decreased rapidly after the mid-exponential phase. These results suggest that the transcription of these two genes is probably regulated by a similar mechanism in response to glucose availability.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Dec
|
pubmed:issn |
0378-1097
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
15
|
pubmed:volume |
181
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
329-36
|
pubmed:dateRevised |
2009-11-19
|
pubmed:meshHeading |
pubmed-meshheading:10585557-Bacterial Proteins,
pubmed-meshheading:10585557-Base Sequence,
pubmed-meshheading:10585557-Blotting, Northern,
pubmed-meshheading:10585557-Clostridium perfringens,
pubmed-meshheading:10585557-Culture Media,
pubmed-meshheading:10585557-Gene Deletion,
pubmed-meshheading:10585557-Genes, Bacterial,
pubmed-meshheading:10585557-Glucose,
pubmed-meshheading:10585557-Hydrogen,
pubmed-meshheading:10585557-Hydrogenase,
pubmed-meshheading:10585557-Iron-Sulfur Proteins,
pubmed-meshheading:10585557-Molecular Sequence Data,
pubmed-meshheading:10585557-Phosphotransferases (Carboxyl Group Acceptor),
pubmed-meshheading:10585557-RNA, Bacterial,
pubmed-meshheading:10585557-Time Factors
|
pubmed:year |
1999
|
pubmed:articleTitle |
The hydA gene encoding the H(2)-evolving hydrogenase of Clostridium perfringens: molecular characterization and expression of the gene.
|
pubmed:affiliation |
Department of Hospital Pharmacy, Faculty of Medicine, Kagawa Medical University, 1750-1, Miki-cho, Kita-gun, Kagawa, Japan.
|
pubmed:publicationType |
Journal Article
|