| pubmed-article:10584030 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10584030 | lifeskim:mentions | umls-concept:C0014279 | lld:lifeskim |
| pubmed-article:10584030 | lifeskim:mentions | umls-concept:C0205360 | lld:lifeskim |
| pubmed-article:10584030 | lifeskim:mentions | umls-concept:C0075351 | lld:lifeskim |
| pubmed-article:10584030 | lifeskim:mentions | umls-concept:C0549178 | lld:lifeskim |
| pubmed-article:10584030 | lifeskim:mentions | umls-concept:C0185125 | lld:lifeskim |
| pubmed-article:10584030 | lifeskim:mentions | umls-concept:C1522492 | lld:lifeskim |
| pubmed-article:10584030 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:10584030 | pubmed:dateCreated | 2000-1-27 | lld:pubmed |
| pubmed-article:10584030 | pubmed:abstractText | Recombinant strains of Pseudomonas putida KT2440 carrying genetic expression cassettes with xylene oxygenase- and styrene monooxygenase-encoding genes on their chromosomes could be induced in shaking-flask experiments to specific activities that rivaled those of multicopy-plasmid-based Escherichia coli recombinants. Such strains maintained the introduced styrene oxidation activity in continuous two-liquid-phase cultures for at least 100 generations, although at a lower level than in the shaking-flask experiments. The data suggest that placement of target genes on the chromosome might be a suitable route for the construction of segregationally stable and highly active whole-cell biocatalysts. | lld:pubmed |
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| pubmed-article:10584030 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10584030 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10584030 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:10584030 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10584030 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:10584030 | pubmed:issn | 0099-2240 | lld:pubmed |
| pubmed-article:10584030 | pubmed:author | pubmed-author:KaiserAA | lld:pubmed |
| pubmed-article:10584030 | pubmed:author | pubmed-author:WitholtBB | lld:pubmed |
| pubmed-article:10584030 | pubmed:author | pubmed-author:de LorenzoVV | lld:pubmed |
| pubmed-article:10584030 | pubmed:author | pubmed-author:WubboltsM GMG | lld:pubmed |
| pubmed-article:10584030 | pubmed:author | pubmed-author:PankeSS | lld:pubmed |
| pubmed-article:10584030 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10584030 | pubmed:volume | 65 | lld:pubmed |
| pubmed-article:10584030 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10584030 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10584030 | pubmed:pagination | 5619-23 | lld:pubmed |
| pubmed-article:10584030 | pubmed:dateRevised | 2010-9-10 | lld:pubmed |
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| pubmed-article:10584030 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10584030 | pubmed:articleTitle | Engineering of a stable whole-cell biocatalyst capable of (S)-styrene oxide formation for continuous two-liquid-phase applications. | lld:pubmed |
| pubmed-article:10584030 | pubmed:affiliation | Institute of Biotechnology, Swiss Federal Institute of Technology Zurich, 8093 Zurich, Switzerland. | lld:pubmed |
| pubmed-article:10584030 | pubmed:publicationType | Journal Article | lld:pubmed |
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