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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2000-1-27
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pubmed:abstractText |
Recombinant strains of Pseudomonas putida KT2440 carrying genetic expression cassettes with xylene oxygenase- and styrene monooxygenase-encoding genes on their chromosomes could be induced in shaking-flask experiments to specific activities that rivaled those of multicopy-plasmid-based Escherichia coli recombinants. Such strains maintained the introduced styrene oxidation activity in continuous two-liquid-phase cultures for at least 100 generations, although at a lower level than in the shaking-flask experiments. The data suggest that placement of target genes on the chromosome might be a suitable route for the construction of segregationally stable and highly active whole-cell biocatalysts.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10584030-10427005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10584030-1366512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10584030-1367010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10584030-1367568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10584030-2172216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10584030-2172217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10584030-6282694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10584030-7487030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10584030-7532480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10584030-7765189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10584030-7798149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10584030-8057911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10584030-8380783,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10584030-8460577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10584030-8979346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10584030-9074492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10584030-9094142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10584030-9119013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10584030-9322742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10584030-9464417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10584030-9603811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10584030-9630987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10584030-9636322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10584030-9748457
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0099-2240
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
65
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5619-23
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pubmed:dateRevised |
2010-9-10
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pubmed:meshHeading |
pubmed-meshheading:10584030-Bacteriological Techniques,
pubmed-meshheading:10584030-Catalysis,
pubmed-meshheading:10584030-DNA Transposable Elements,
pubmed-meshheading:10584030-Epoxy Compounds,
pubmed-meshheading:10584030-Escherichia coli,
pubmed-meshheading:10584030-Genetic Engineering,
pubmed-meshheading:10584030-Kinetics,
pubmed-meshheading:10584030-Mixed Function Oxygenases,
pubmed-meshheading:10584030-Mutagenesis, Insertional,
pubmed-meshheading:10584030-Oxidation-Reduction,
pubmed-meshheading:10584030-Oxygenases,
pubmed-meshheading:10584030-Pseudomonas putida,
pubmed-meshheading:10584030-Recombination, Genetic,
pubmed-meshheading:10584030-Transformation, Bacterial
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pubmed:year |
1999
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pubmed:articleTitle |
Engineering of a stable whole-cell biocatalyst capable of (S)-styrene oxide formation for continuous two-liquid-phase applications.
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pubmed:affiliation |
Institute of Biotechnology, Swiss Federal Institute of Technology Zurich, 8093 Zurich, Switzerland.
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pubmed:publicationType |
Journal Article
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