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rdf:type |
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lifeskim:mentions |
umls-concept:C0025260,
umls-concept:C0032659,
umls-concept:C0039195,
umls-concept:C0054965,
umls-concept:C0085358,
umls-concept:C0086418,
umls-concept:C1332717,
umls-concept:C1413244,
umls-concept:C1516670,
umls-concept:C1706438,
umls-concept:C2698600
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pubmed:issue |
3
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pubmed:dateCreated |
2000-1-4
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pubmed:abstractText |
The proportion of human peripheral blood CD8+ T cells that are CD57+ CD28- is low at birth but increases with age and in individuals infected with human cytomegalovirus (HCMV) or human immunodeficiency virus (HIV). These CD57+ CD28- CD8+ T cells contain large oligoclonal T-cell expansions whose antigen specificity is unknown. We identified clonal expansions of virus-specific memory cytotoxic T-lymphocyte precursors (CTLp) in both healthy carriers of HCMV and in asymptomatic HIV-infected subjects. In each subject, from the T-cell receptor (TCR) beta-chain hypervariable sequence of each immunodominant CTL clone, we designed complementary oligonucleotide probes to quantify the size and phenotypic segregation of individual virus-specific CTL clones in highly purified populations of peripheral blood CD8+ T cells. We found large clonal expansions of virus-specific CTL clonotypes in CD57+ CD28- CD8+ T cells. Using limiting dilution analysis, we found functional peptide-specific CTLp at high frequency in CD57+ CD28- cells. Thus, memory CTL specific for persistent viruses account for many oligoclonal expansions within CD57+ CD28- CD8+ T cells.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-10358151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-10358214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-2419407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-2852081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-2947844,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-3097061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-3104390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-3156193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-6226586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-6328614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-7054358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-7511079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-7515928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-7517872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-7522010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-7538544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-7539725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-7594513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-7675046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-7690806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-7693383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-8011246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-8093890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-8294871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-8381831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-8892876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-9842899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10583606-9971792
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
98
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
443-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10583606-Antigens, CD28,
pubmed-meshheading:10583606-Antigens, CD57,
pubmed-meshheading:10583606-CD8-Positive T-Lymphocytes,
pubmed-meshheading:10583606-Clone Cells,
pubmed-meshheading:10583606-Cytomegalovirus,
pubmed-meshheading:10583606-Cytomegalovirus Infections,
pubmed-meshheading:10583606-Flow Cytometry,
pubmed-meshheading:10583606-HIV Infections,
pubmed-meshheading:10583606-Humans,
pubmed-meshheading:10583606-Immunologic Memory,
pubmed-meshheading:10583606-Immunophenotyping,
pubmed-meshheading:10583606-T-Lymphocyte Subsets,
pubmed-meshheading:10583606-T-Lymphocytes, Cytotoxic
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pubmed:year |
1999
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pubmed:articleTitle |
Large clonal expansions of human virus-specific memory cytotoxic T lymphocytes within the CD57+ CD28- CD8+ T-cell population.
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pubmed:affiliation |
Department of Medicine, University of Cambridge Clinical School, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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