Source:http://linkedlifedata.com/resource/pubmed/id/10579091
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
1999-12-15
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| pubmed:abstractText |
Six Candida dubliniensis isolates were recovered from two HIV-infected individuals in the course of a prospective study of recurrent oral candidosis among HIV-positive patients in Spain. Candida albicans strains as well as non-albicans strains were also obtained from these two patients. C. dubliniensis strains were germ-tube-positive and produced abundant chlamydospores. Fingerprinting the genomic DNAs of these six C. dubliniensis with the C. albicans-specific probe 27A as well as karyotyping was performed to confirm the identification of these isolates. Further analysis of their genomic DNAs was performed by PCR-fingerprinting with the core sequence of phage M13, and they exhibited species-specific multilocus band patterns, clearly distinct from those of C. albicans isolates analyzed in this study and in a previous one (Diaz-Guerra 1997). Intraspecies variation was also seen among PCR patterns yielded by C. dubliniensis isolates from different patients. Although few strains have been analyzed, the use of this PCR-fingerprinting procedure is a promising tool for further epidemiologic studies with C. dubliniensis. The isolation of C. dubliniensis from Spanish HIV-infected patients contributes to the idea of widespread geographic distribution of this species.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
0732-8893
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
35
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
113-9
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| pubmed:dateRevised |
2004-11-17
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| pubmed:meshHeading |
pubmed-meshheading:10579091-Candida,
pubmed-meshheading:10579091-DNA Fingerprinting,
pubmed-meshheading:10579091-HIV Seropositivity,
pubmed-meshheading:10579091-Humans,
pubmed-meshheading:10579091-Microbial Sensitivity Tests,
pubmed-meshheading:10579091-Polymerase Chain Reaction,
pubmed-meshheading:10579091-Prospective Studies
|
| pubmed:year |
1999
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| pubmed:articleTitle |
Molecular characterization by PCR-fingerprinting of Candida dubliniensis strains isolated from two HIV-positive patients in Spain.
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| pubmed:affiliation |
Unidad de Micología, Centro Nacional de Microbiología, Majadahonda, Madrid, Spain.
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| pubmed:publicationType |
Journal Article
|