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rdf:type |
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lifeskim:mentions |
umls-concept:C0007586,
umls-concept:C0007634,
umls-concept:C0032334,
umls-concept:C0086243,
umls-concept:C0086418,
umls-concept:C0229613,
umls-concept:C0441472,
umls-concept:C0733482,
umls-concept:C0733689,
umls-concept:C2603343,
umls-concept:C2828360
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pubmed:issue |
3
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pubmed:dateCreated |
1975-11-22
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pubmed:abstractText |
Flow microfluorometric analysis of human lymphoid cells exposed in vitro to cytostatic concentrations of podophyllotoxin (0.01-5 mug/ml for 24 h) shows that a major part of this population (40-60%) has the DNA content of cells in the G2-M part of the cell cycle, and that approximately 60% of these cells are arrested in mitosis. Although a similar pattern of DNA distribution is seen in cultures exposed to cytostatic concentrations of VM-26(0.01 mug/ml) and VP--16-213(0.1 mug/ml), no mitotic cells are seen in these cultures. Exposure to higher concentrations: of VM-26 (0.1 mug/ml) and VP-16-213 (1.0 mug/ml) inhibits cell cycle traverse, and after 24 hr of exposure a major part of the population is arrested with the DNA content of cell in the S part of the cell cycle. Exposure to higher drug concentrations leads to a reduction in the number of cells with the late S-G2DNA content. Whereas the cell cycle block induced by cytostatic concentrations of podophyllotoxin (0.01 mug/ml) is readily reversible by reincubation of cells in drug-free medium, cells blocked by VM-26 and VP-16-213 are unable to resume cell-cycle traverse under similar conditions.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1057547-13233838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1057547-14189913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1057547-14278051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1057547-14326124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1057547-14819869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1057547-17752486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1057547-4210356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1057547-4321351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1057547-4342528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1057547-4855812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1057547-49354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1057547-4991932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1057547-5279516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1057547-5445836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1057547-5559844,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1057547-5671289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1057547-5678448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1057547-5689568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1057547-5707852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1057547-5782454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1057547-5827239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1057547-5892129
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0021-9525
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
66
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
521-30
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pubmed:dateRevised |
2010-9-3
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pubmed:meshHeading |
pubmed-meshheading:1057547-Cell Count,
pubmed-meshheading:1057547-Cell Division,
pubmed-meshheading:1057547-Cell Line,
pubmed-meshheading:1057547-Cell Survival,
pubmed-meshheading:1057547-DNA,
pubmed-meshheading:1057547-Dose-Response Relationship, Drug,
pubmed-meshheading:1057547-Fluorometry,
pubmed-meshheading:1057547-Humans,
pubmed-meshheading:1057547-Leukemia, Lymphoid,
pubmed-meshheading:1057547-Lymphocytes,
pubmed-meshheading:1057547-Mitosis,
pubmed-meshheading:1057547-Mitotic Index,
pubmed-meshheading:1057547-Podophyllotoxin
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pubmed:year |
1975
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pubmed:articleTitle |
Cytofluorometric studies on the action of podophyllotoxin and epipodophyllotoxins (VM-26, VP-16-213) on the cell cycle traverse of human lymphoblasts.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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