Linked Life Data

10574715

Source:http://linkedlifedata.com/resource/pubmed/id/10574715

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2000-4-24
pubmed:abstractText
Microtubule motors, such as the minus end-directed motor, cytoplasmic dynein, play an important role in maintaining the integrity, intracellular location, and function of the Golgi apparatus, as well as in the translocation of membrane between the endoplasmic reticulum and Golgi apparatus. We have immunolocalised conventional cytoplasmic dynein heavy chain to the Golgi apparatus in cultured vertebrate cells. In addition, we present evidence that cytoplasmic dynein heavy chain cycles constitutively between the endoplasmic reticulum and Golgi apparatus: it colocalises partially with the intermediate compartment, it is found on nocodazole-induced peripheral Golgi elements and, most strikingly, on Brefeldin A-induced tubules that are moving towards microtubule plus ends. The direction of movement of membrane between the endoplasmic reticulum and Golgi apparatus is therefore unlikely to be regulated by controlling motor-membrane interactions: rather, the motors probably remain bound throughout the whole cycle, with their activity being modulated instead. We also report that the overexpression of p50/dynamitin results in the loss of cytoplasmic dynein heavy chain from the membrane of peripheral Golgi elements. These results explain how dynamitin overexpression causes the inhibition of endoplasmic reticulum-to-Golgi transport complex movement towards the centrosomal region, and support the general model that an intact dynactin complex is required for cytoplasmic dynein binding to all cargoes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9533
pubmed:author
pubmed:issnType
Print
pubmed:volume
112 ( Pt 24)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4673-85
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Dynamic association of cytoplasmic dynein heavy chain 1a with the Golgi apparatus and intermediate compartment.
pubmed:affiliation
School of Biological Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't