Linked Life Data

10574211

Source:http://linkedlifedata.com/resource/pubmed/id/10574211

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-12-2
pubmed:abstractText
A method of precolumn derivatization GC with FID detection was developed for a simultaneous analysis of five major steroidal alkaloids of Fritillaria species, namely ebeiedine, ebeiedinone, verticine, verticinone and imperialine. Derivatization was carried out by trimethylsilylation of the hydroxyl-containing Fritillaria alkaloids to the corresponding trimethylsilylates with trimethylsilylimidazole. Reaction conditions were optimised and the alkaloids derivatives were characterised by on-line GC-MS. The validated GC method demonstrated a good linearity at the sampling ranges used. This analytical method is simple, convenient and reproducible. The developed assay was successfully applied to the determination of the major pharmacologically active alkaloids in three commonly used antitussive Fritillaria species: F. cirrhosa, F. thunbergii and F. pallidiflora.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
29
pubmed:volume
859
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
183-92
pubmed:dateRevised
2009-1-15
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Pre-column derivatization and gas chromatographic determination of alkaloids in bulbs of Fritillaria.
pubmed:affiliation
Department of Pharmacognosy, China Pharmaceutical University, Nanjing.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't